4-Nitrophenyl phosphate disodium salt hexahydrate
- CAS No.
- 333338-18-4
- Chemical Name:
- 4-Nitrophenyl phosphate disodium salt hexahydrate
- Synonyms
- PNPP-NA;PHOSPHATASE SUBSTRATE;P-NITROPHENYL PHOSPHATE DISODIUM HEXAHYDRATE;P-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE;t hexahydrate;PNPP hexahydrate;pNPP-Na hexahydrate;PNPP DISODIUM HEXAHYDRATE;PNP-PHOS DINA HEXAHYDRATE;Phosphatase substrate【P4744】
- CBNumber:
- CB4681371
- Molecular Formula:
- C6H9NNaO7P
- Molecular Weight:
- 261.1
- MDL Number:
- MFCD00066288
- MOL File:
- 333338-18-4.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | >300 °C(lit.) |
|---|---|
| storage temp. | -20°C |
| solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| form | tablets |
| color | white to pale yellow |
| PH | pH (50g/l, 25℃) : 8.0~9.0 |
| biological source | synthetic (organic) |
| Water Solubility | almost transparency |
| BRN | 5324661 |
| InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 |
| InChIKey | KAKKHKRHCKCAGH-UHFFFAOYSA-L |
| SMILES | O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1 |
| CAS DataBase Reference | 333338-18-4(CAS DataBase Reference) |
| UNSPSC Code | 12352204 |
| NACRES | NA.83 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) | ![]() GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335 | |||||||||
| Precautionary statements | P261-P271-P280 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Safety Statements | 24/25 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 8-10-21 | |||||||||
| HS Code | 29199000 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| NFPA 704 |
|
4-Nitrophenyl phosphate disodium salt hexahydrate price More Price(99)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | S0942 | Phosphatase substrate 5 mg tablets | 333338-18-4 | 50 tabs | $63 | 2026-04-30 | Buy |
| Sigma-Aldrich | S0942 | Phosphatase substrate 5 mg tablets | 333338-18-4 | 100 tabs | $112 | 2026-04-30 | Buy |
| Sigma-Aldrich | S0942 | Phosphatase substrate 5 mg tablets | 333338-18-4 | 200 tabs | $197 | 2026-04-30 | Buy |
| Sigma-Aldrich | P5994 | Phosphatase substrate 40 mg tablets | 333338-18-4 | 25 tabs | $193 | 2026-04-30 | Buy |
| Sigma-Aldrich | P5869 | Phosphatase substrate 100 mg capsules | 333338-18-4 | 10caps | $234 | 2026-04-30 | Buy |
4-Nitrophenyl phosphate disodium salt hexahydrate Chemical Properties, Uses, Production
Chemical Properties
White to off-white powder
Uses
Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
Uses
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
Uses
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
General Description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
4-Nitrophenyl phosphate disodium salt hexahydrate Preparation Products And Raw materials
Raw materials
Preparation Products
4-Nitrophenyl phosphate disodium salt hexahydrate Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Guangzhou Yaoguang Technology Co., Ltd. | 13020159086 | yangjue@ygfuture.com | China | 101 | 58 |
| Nanjing UltraPure Biotech Co., Ltd. | 025-82263158 13851993283 | 2842979086@qq.com | China | 243 | 58 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Adamas Reagent, Ltd. | 400-6009262 15618770481 | yhx@titansci.com | China | 14098 | 59 |
| Tianjin heowns Biochemical Technology Co., Ltd. | 400 638 7771 | sales@heowns.com | China | 14412 | 57 |
| Sinopharm Chemical Reagent Co,Ltd. | 86-21-63210123 | sj_scrc@sinopharm.com | China | 9791 | 79 |
| Hunan Hui Bai Shi Biotechnology Co., Ltd. | 0731-85526065 13308475853 | ivy@hnhbsj.com | China | 7236 | 62 |
View Latest Price from 4-Nitrophenyl phosphate disodium salt hexahydrate manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
![]() |
2026-07-15 | Sodium 4-nitrophenyl phosphate hexahydrate
333338-18-4
|
$40.00 | 99.81% | 10g | TargetMol Chemicals Inc. | ||
![]() |
2026-06-30 | PNPP
333338-18-4
|
1kg | 98% | 1000 | Shaanxi Dideu New Materials Co. Ltd | ||
![]() |
2021-07-13 | 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
333338-18-4
|
$10.00-15.00 | 1KG | 99%+ HPLC | Zhuozhou Wenxi import and Export Co., Ltd |
-

- Sodium 4-nitrophenyl phosphate hexahydrate
333338-18-4
- $40.00
- 99.81%
- TargetMol Chemicals Inc.
-

- PNPP
333338-18-4
- 98%
- Shaanxi Dideu New Materials Co. Ltd
-

- 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
333338-18-4
- $10.00-15.00
- 99%+ HPLC
- Zhuozhou Wenxi import and Export Co., Ltd





