BCECF-AM
- CAS No.
- 117464-70-7
- Chemical Name:
- BCECF-AM
- Synonyms
- BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF AM pH Indicator (Green);BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN
- CBNumber:
- CB5111514
- Molecular Formula:
- C42H40O21
- Molecular Weight:
- 880.75
- MDL Number:
- MFCD00036969
- MOL File:
- 117464-70-7.mol
| Product description | Number | Pack Size | Price |
| 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 1 mg/mL in DMSO | B8806 | 1 mg | $296 |
| BCECF/AM | 216254 | 1mg | $199 |
| BCECF-Acetoxymethyl ester ≥95% | 15922 | 1mg | $117 |
| BCECF-Acetoxymethyl ester ≥95% | 15922 | 5mg | $521 |
| BCECF-Acetoxymethyl ester ≥95% | 15922 | 25mg | $1540 |
| Boiling point | 692.41°C (rough estimate) |
|---|---|
| Density | 1.2837 (rough estimate) |
| refractive index | 1.6500 (estimate) |
| Flash point | 85 °C |
| storage temp. | -20°C |
| solubility |
acetonitrile: 25mg/mL DMF: soluble DMSO: soluble |
| form | White to off-white solid |
| color | Orange to red |
| Appearance | white powder |
| Major Application |
diagnostic assay manufacturing hematology histology |
| InChIKey | NTECHUXHORNEGZ-UHFFFAOYSA-N |
| SMILES | CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O |
| CAS DataBase Reference | 117464-70-7(CAS DataBase Reference) |
| UNSPSC Code | 12352200 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H319-H315-H335 |
| Precautionary statements | P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 22-26-36 |
| WGK Germany | 3 |
| Storage Class | 10 - Combustible liquids |
BCECF-AM price More Price(15)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | B8806 | 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 1 mg/mL in DMSO | 1 mg | $296 | 2025-07-31 | Buy | |
| Sigma-Aldrich | 216254 | BCECF/AM | 117464-70-7 | 1mg | $199 | 2025-07-31 | Buy |
| Cayman Chemical | 15922 | BCECF-Acetoxymethyl ester ≥95% | 117464-70-7 | 1mg | $117 | 2024-03-01 | Buy |
| Cayman Chemical | 15922 | BCECF-Acetoxymethyl ester ≥95% | 117464-70-7 | 5mg | $521 | 2024-03-01 | Buy |
| Cayman Chemical | 15922 | BCECF-Acetoxymethyl ester ≥95% | 117464-70-7 | 25mg | $1540 | 2021-12-16 | Buy |
BCECF-AM Chemical Properties,Uses,Production
Description
BCECF AM ester is a widely-
Chemical Properties
pale yellow to yellow powder
Uses
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
General Description
Material is an isomeric mixture of lactones, acetates, and AM esters
Biological Activity
Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.
References
[1] T J RINK T P R Y Tsien. Cytoplasmic pH and free Mg2+ in lymphocytes.[J]. Journal of Cell Biology, 1982, 95 1: 189-196. DOI: 10.1083/jcb.95.1.189
[2] PINAR OZKAN Raj M. A rapid method for measuring intracellular pH using BCECF-AM[J]. Biochimica et biophysica acta. General subjects, 2002, 1572 1: Pages 143-148. DOI: 10.1016/s0304-4165(02)00303-3
[3] CAROLINE DIVE. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases[J]. Molecular and Cellular Probes, 1988, 2 2: Pages 131-145. DOI: 10.1016/0890-8508(88)90035-7
[4] NATHAN O’CONNOR Randi B S. Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells.[J]. Methods in cell biology, 2013, 114: 387-406. DOI: 10.1016/b978-0-12-407761-4.00016-6
[5] SUE CHOW Ian T David Hedley. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases[J]. Cytometry Part B: Clinical Cytometry, 1996, 24 4: 360-367. DOI: 10.1002/(sici)1097-0320(19960801)24:4<360::aid-cyto7>3.0.co;2-j
[6] LáSZLó BALKAY. Flow cytometric determination of intracellular free potassium concentration[J]. Cytometry Part B: Clinical Cytometry, 1998, 28 1: 42-49. DOI: 10.1002/(sici)1097-0320(19970501)28:1<42::aid-cyto5>3.0.co;2-j
Abs/Em Maxima
440; 490/530 nm
Excitation / Emission Maximum
440-490 nm/535 nm
BCECF-AM Preparation Products And Raw materials
Raw materials
Preparation Products
BCECF-AM Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49977 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354; +17819995354 | marketing@targetmol.com | United States | 32469 | 58 |
| Finetech Industry Limited | +86-27-8746-5837 +8619945049750 | info@finetechnology-ind.com | China | 9629 | 58 |
| HANGZHOU LEAP CHEM CO., LTD. | +86-571-87711850 | market18@leapchem.com | China | 43333 | 58 |
| Aladdin Scientific | tp@aladdinsci.com | United States | 57505 | 58 | |
| Aladdin Scientific | tp@aladdinsci.com | United States | 52923 | 58 | |
| DAYANG CHEM (HANGZHOU) CO.,LTD | +86-88938639 +86-17705817739 | info@dycnchem.com | China | 53803 | 58 |
| ANHUI BYERSEN BIOTECH CO. LTD | +86-15385096007 +86-17755058861 | amy@byersenchemical.com | China | 10000 | 58 |
| NANJING YINGWEN BIOTECHNOLOGY CO LTD | +8613382787392 | sales@aeechem.com | China | 9999 | 58 |
| Mainchem Co., Ltd. | +86-0592-6210733 | sale@mainchem.com | China | 32343 | 55 |
