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BCECF-AM

BCECF-AM structure

CAS No.: 117464-70-7

Chemical Name:: BCECF-AM

Synonyms: BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF AM pH Indicator (Green);BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN

CBNumber:: CB5111514

Molecular Formula:: C42H40O21

Molecular Weight:: 880.75

MDL Number:: MFCD00036969

MOL File:: 117464-70-7.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-05-28 02:10:58

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BCECF-AM Properties

Boiling point	692.41°C (rough estimate)
Density	1.2837 (rough estimate)
refractive index	1.6500 (estimate)
Flash point	85 °C
storage temp.	-20°C
solubility	acetonitrile: 25mg/mL DMF: soluble DMSO: soluble
form	White to off-white solid
color	Orange to red
Appearance	white powder
Major Application	diagnostic assay manufacturing hematology histology
InChIKey	NTECHUXHORNEGZ-UHFFFAOYSA-N
SMILES	CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O
CAS DataBase Reference	117464-70-7(CAS DataBase Reference)
UNSPSC Code	12352200
NACRES	NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H319-H315-H335
Precautionary statements	P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	22-26-36
WGK Germany	3
Storage Class	10 - Combustible liquids

BCECF-AM price More Price(20)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	B8806	2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 1 mg/mL in DMSO		1 mg	$215.6	2026-04-30	Buy
Sigma-Aldrich	216254	BCECF/AM	117464-70-7	1mg	$213	2026-03-19	Buy
Cayman Chemical	15922	BCECF-Acetoxymethyl ester ≥95%	117464-70-7	1mg	$117	2024-03-01	Buy
Cayman Chemical	15922	BCECF-Acetoxymethyl ester ≥95%	117464-70-7	5mg	$521	2024-03-01	Buy
Usbiological	263576	2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	117464-70-7	10mg	$3787	2026-06-03	Buy

Product number	Packaging	Price	Buy
B8806	1 mg	$215.6	Buy
216254	1mg	$213	Buy
15922	1mg	$117	Buy
15922	5mg	$521	Buy
263576	10mg	$3787	Buy

BCECF-AM Chemical Properties,Uses,Production

Description

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Chemical Properties

pale yellow to yellow powder

Uses

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

General Description

Material is an isomeric mixture of lactones, acetates, and AM esters

Biological Activity

Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.

References

[1] T J RINK T P R Y Tsien. Cytoplasmic pH and free Mg2+ in lymphocytes.[J]. Journal of Cell Biology, 1982, 95 1: 189-196. DOI: 10.1083/jcb.95.1.189
[2] PINAR OZKAN Raj M. A rapid method for measuring intracellular pH using BCECF-AM[J]. Biochimica et biophysica acta. General subjects, 2002, 1572 1: Pages 143-148. DOI: 10.1016/s0304-4165(02)00303-3
[3] CAROLINE DIVE. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases[J]. Molecular and Cellular Probes, 1988, 2 2: Pages 131-145. DOI: 10.1016/0890-8508(88)90035-7
[4] NATHAN O’CONNOR Randi B S. Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells.[J]. Methods in cell biology, 2013, 114: 387-406. DOI: 10.1016/b978-0-12-407761-4.00016-6
[5] SUE CHOW Ian T David Hedley. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases[J]. Cytometry Part B: Clinical Cytometry, 1996, 24 4: 360-367. DOI: 10.1002/(sici)1097-0320(19960801)24:4<360::aid-cyto7>3.0.co;2-j
[6] LáSZLó BALKAY. Flow cytometric determination of intracellular free potassium concentration[J]. Cytometry Part B: Clinical Cytometry, 1998, 28 1: 42-49. DOI: 10.1002/(sici)1097-0320(19970501)28:1<42::aid-cyto5>3.0.co;2-j

Abs/Em Maxima

440; 490/530 nm

Excitation / Emission Maximum

440-490 nm/535 nm

BCECF-AM Preparation Products And Raw materials

Raw materials

Preparation Products

BCECF-AM Suppliers

Global( 184)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
Beijing Ouhe Technology Co., Ltd	13552068683 13522913783	2355560935@qq.com	China	11777	60
Dalian Meilun Biotech Co., Ltd.	0411-62910999 13889544652	sales@meilune.com	China	4765	58
ShangHai YuanYe Biotechnology Co., Ltd.	15026964105	shyysw007@163.com	China	4998	60
Artis Chemistry (Shanghai) Co. Ltd.	86-21-60936353		China	335	58
Thermo Fisher Scientific	800-810-5118	cnchemical@thermofisher.com	China	17752	75
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-6206333 13167063860	anhua.mao@aladdin-e.com	China	29985	65
Chengdu HuaXia Chemical Reagent Co. Ltd	400-1166-196 13458535857	cdhxsj@163.com	China	13323	58
Finetech Industry Limited	027-87465837 19945049750	sales@finetechnology-ind.com	China	9335	58
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51389	80

Supplier	Advantage
3B Pharmachem (Wuhan) International Co.,Ltd.	69
Beijing Ouhe Technology Co., Ltd	60
Dalian Meilun Biotech Co., Ltd.	58
ShangHai YuanYe Biotechnology Co., Ltd.	60
Artis Chemistry (Shanghai) Co. Ltd.	58
Thermo Fisher Scientific	75
Shanghai Aladdin Bio-Chem Technology Co.,LTD	65
Chengdu HuaXia Chemical Reagent Co. Ltd	58
Finetech Industry Limited	58
Sigma-Aldrich	80

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2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO Bcecf-AmSolution 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers bcecf-acetoxymethyl 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein AM ester BCECF-AM solution, Spiro(isobenzofuran-1(3H),9μ-(9H)xanthene)-2μ,7μ-dipropanoic acid 2',7'-Bis(2-acetoxymethoxycarbonylethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid acetoxymethyl ester 2,7-Bis[2-(acetoxymethoxycarbonyl)ethyl]-3,6-dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-6'-carboxylic acid acetoxymethyl ester 2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester BCECF-AM 3',6'-bis(Acetyloxy)-5(or6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid2',7'-bis[(acetyloxy)methyl]ester BCECF/AM - CAS 117464-70-7 - Calbiochem BCECF-Acetoxymethyl ester BCECF--1M BCECF--1M 1MG SPIRO(ISOBENZOFURAN-1(3H),9'-(9H)XANTHENE)-2',7'-DIPROPANOIC ACID 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxyMet 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethy 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester BCECF-AM, intracellular ratiometric pH indicator BCECF AM pH Indicator (Green) 1H-Benz[e]indolium, 3-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-2-[2-[3-[2-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,1-dimethyl-, chloride (1:1) (ACI) 117464-70-7 C42H40O21 C39H36O19 Cell Signaling and Neuroscience BioChemical Cell Biology Ion Probes Ion Channels Monovalent Ion Channels Voltage-gated Ion Channels