ChemicalBook >> CAS DataBase List >>5-CARBOXYFLUORESCEIN DIACETATE
5-CARBOXYFLUORESCEIN DIACETATE
- CAS No.
- 79955-27-4
- Chemical Name:
- 5-CARBOXYFLUORESCEIN DIACETATE
- Synonyms
- CFDA;5-FAM DA;5-diacetate FAM;5(6)-FAM DIACETATE;5-CFDA, 10 mM in DMSO;5-carboxydiacetic acidester;5-Carboxyfluorescein diacetate;5-carboxy' Uorescein Diacetate;CARBOXYFLUORESCEIN DI-O-ACETATE;5-Carboxyfluorescein Diacetate >
- CBNumber:
- CB9669200
- Molecular Formula:
- C25H16O9
- Molecular Weight:
- 460.39
- MDL Number:
- MFCD00036872
- MOL File:
- 79955-27-4.mol
- MSDS File:
- SDS
- TDS File:
- TDS
Last updated:2026-04-22 18:57:36
| Product description | Number | Pack Size | Price |
| 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem | 216275-M | 50mg | $250 |
| 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem | 216275-M | 216275-50MG | $138 |
| 5-Carboxyfluorescein diacetate ~95% (HPLC) | C4916 | 25mg | $104 |
| 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) | C2859 | 50mg | $74 |
| 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) | C2859 | 200mg | $218 |
| More product size | |||
| Melting point | >250°C |
|---|---|
| Boiling point | 693.1±55.0 °C(Predicted) |
| Density | 1.57±0.1 g/cm3(Predicted) |
| storage temp. | Sealed in dry,Store in freezer, under -20°C |
| solubility | DMSO: soluble |
| pka | 6.4(at 25℃) |
| form | Solid |
| color | Colorless solid |
| PH Range | Weak green ' uorescence (6.0) to strong green ' uourescence (7.2) |
| λmax | <300nm |
| BRN | 68265 |
| Major Application | Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs |
| Biological Applications | Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity |
| InChI | 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
| InChIKey | WPUZGNPQMIWOHE-UHFFFAOYSA-N |
| SMILES | CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1 |
| UNSPSC Code | 41116134 |
| NACRES | NA.51 |
SAFETY
Risk and Safety Statements
| PPE | Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges |
|---|---|
| Hazard Codes | T |
| Risk Statements | 45-46-23/24/25-36/37/38 |
| Safety Statements | 53-22-26-36/37/39-45 |
| WGK Germany | 3 |
| F | 10-21 |
| HS Code | 32049010 |
| Storage Class | 11 - Combustible Solids |
5-CARBOXYFLUORESCEIN DIACETATE price More Price(75)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 216275-M | 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem | 79955-27-4 | 50mg | $250 | 2026-04-30 | Buy |
| Sigma-Aldrich | 216275-M | 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem | 79955-27-4 | 216275-50MG | $138 | 2024-03-01 | Buy |
| Sigma-Aldrich | C4916 | 5-Carboxyfluorescein diacetate ~95% (HPLC) | 79955-27-4 | 25mg | $104 | 2023-06-20 | Buy |
| TCI Chemical | C2859 | 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) | 79955-27-4 | 50mg | $74 | 2026-04-30 | Buy |
| TCI Chemical | C2859 | 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) | 79955-27-4 | 200mg | $218 | 2026-04-30 | Buy |
5-CARBOXYFLUORESCEIN DIACETATE Chemical Properties,Uses,Production
Uses
Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein.
Uses
5-Carboxyfluorescein Diacetate is used in the synthesis of Rhodamines. Dyes and metabolites.
Biological Activity
Primary Target
Vital stain
5-CARBOXYFLUORESCEIN DIACETATE Preparation Products And Raw materials
Raw materials
Preparation Products
Global( 167)Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Nanjing ChemLin Chemical Industry Co., Ltd. | 025-83697070 | product@chemlin.com.cn | CHINA | 3009 | 60 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49979 | 58 |
| career henan chemical co | +86-0371-86658258 +8613203830695 | factory@coreychem.com | China | 29792 | 58 |
| SIMAGCHEM CORP | +86-5922680277 +86-13806087780 | sale@simagchem.com | China | 17346 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354; +17819995354 | marketing@targetmol.com | United States | 32466 | 58 |
| BOC Sciences | 16314854226; +16314854226 | inquiry@bocsci.com | United States | 19853 | 58 |
| Dayang Chem (Hangzhou) Co.,Ltd. | +86-0571-88938639 +8617705817739 | info@dycnchem.com | China | 52705 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418684 +8618949823763 | sales@tnjchem.com | China | 25356 | 58 |
| Hebei Chuanghai Biotechnology Co., Ltd | +8615531151365 | mina@chuanghaibio.com | China | 18126 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354; | support@targetmol.com | United States | 39039 | 58 |
View Lastest Price from 5-CARBOXYFLUORESCEIN DIACETATE manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
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2026-04-22 | 5-CFDA
79955-27-4
|
$33.00-47.00 | 98.39% | 10g | TargetMol Chemicals Inc. | ||
![]() |
2026-04-22 | 5-CFDA
79955-27-4
|
$33.00-47.00 | 98.39% | 10g | TargetMol Chemicals Inc. | ||
![]() |
2025-06-07 | 5-diacetate FAM
79955-27-4
|
1g | >95.00% | 1g | Xian confluore Biological Technology Co., Ltd. |
-

- 5-CFDA
79955-27-4
- $33.00-47.00
- 98.39%
- TargetMol Chemicals Inc.
-

- 5-CFDA
79955-27-4
- $33.00-47.00
- 98.39%
- TargetMol Chemicals Inc.
-

- 5-diacetate FAM
79955-27-4
- $0.00
- >95.00%
- Xian confluore Biological Technology Co., Ltd.
79955-27-4(5-CARBOXYFLUORESCEIN DIACETATE)Related Search:
5(6)-CFDA
5-CARBOXYFLUORESCEIN DIACETATE
5-CFDA N-succinimidyl ester
BCECF-AM
5(6)-CARBOXYFLUORESCEIN DIISOBUTYRATE
6-CARBOXYFLUORESCEIN DIACETATE
6-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
6-Carboxyfluorescein
Fluorescein diacetate
1of4
5-Carboxy-di-O-acetylfluorescein, 5-CFDA
5-FAM DA
3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid
5-Carboxyfluorescein diacetate
3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
5-CFDA [5-Carboxyfluorescein diacetate]
5-Carboxyfluorescein diacetate≥ 95% (HPLC)
BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC)
CARBOXYFLUORESCEIN DI-O-ACETATE
5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE
5(6)-FAM DIACETATE
5(6)-CARBOXY-DI-O-ACETYLFLUORESCEIN
5-carboxy' Uorescein Diacetate
5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem
5-Carboxyfluorescein Diacetate >
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-
5-diacetate FAM
5-CFDA,5CFDA,Inhibitor,inhibit,5 CFDA
5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate
5-CFDA, 10 mM in DMSO
5(6)-Carboxyfluorescein diacetate, solution, DMSO solution
5-carboxydiacetic acidester
CFDA
79955-27-4
2C25H16O9
Biochemicals and Reagents
BioChemical
Fluorescent Probes, Labels, Particles and Stains
Detection
marker
Aromatics
Heterocycles
Intermediates & Fine Chemicals
Pharmaceuticals
Fluorescent
Xanthene






