ChemicalBook >> CAS DataBase List >>5-CARBOXYFLUORESCEIN DIACETATE

5-CARBOXYFLUORESCEIN DIACETATE

CAS No.
79955-27-4
Chemical Name:
5-CARBOXYFLUORESCEIN DIACETATE
Synonyms
CFDA;5-FAM DA;5-diacetate FAM;5(6)-FAM DIACETATE;5-CFDA, 10 mM in DMSO;5-carboxydiacetic acidester;5-Carboxyfluorescein diacetate;5-carboxy' Uorescein Diacetate;CARBOXYFLUORESCEIN DI-O-ACETATE;5-Carboxyfluorescein Diacetate >
CBNumber:
CB9669200
Molecular Formula:
C25H16O9
Molecular Weight:
460.39
MDL Number:
MFCD00036872
MOL File:
79955-27-4.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-04-22 18:57:36
Product description Number Pack Size Price
5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem 216275-M 50mg $250
5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem 216275-M 216275-50MG $138
5-Carboxyfluorescein diacetate ~95% (HPLC) C4916 25mg $104
5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) C2859 50mg $74
5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) C2859 200mg $218
More product size

5-CARBOXYFLUORESCEIN DIACETATE Properties

Melting point >250°C
Boiling point 693.1±55.0 °C(Predicted)
Density 1.57±0.1 g/cm3(Predicted)
storage temp. Sealed in dry,Store in freezer, under -20°C
solubility DMSO: soluble
pka 6.4(at 25℃)
form Solid
color Colorless solid
PH Range Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
λmax <300nm
BRN 68265
Major Application Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs
Biological Applications Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity
InChI 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
InChIKey WPUZGNPQMIWOHE-UHFFFAOYSA-N
SMILES CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1
UNSPSC Code 41116134
NACRES NA.51

SAFETY

Risk and Safety Statements

PPE Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Hazard Codes  T
Risk Statements  45-46-23/24/25-36/37/38
Safety Statements  53-22-26-36/37/39-45
WGK Germany  3
10-21
HS Code  32049010
Storage Class 11 - Combustible Solids

5-CARBOXYFLUORESCEIN DIACETATE price More Price(75)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 216275-M 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem 79955-27-4 50mg $250 2026-04-30 Buy
Sigma-Aldrich 216275-M 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem 79955-27-4 216275-50MG $138 2024-03-01 Buy
Sigma-Aldrich C4916 5-Carboxyfluorescein diacetate ~95% (HPLC) 79955-27-4 25mg $104 2023-06-20 Buy
TCI Chemical C2859 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) 79955-27-4 50mg $74 2026-04-30 Buy
TCI Chemical C2859 5-Carboxyfluorescein Diacetate >93.0%(HPLC)(T) 79955-27-4 200mg $218 2026-04-30 Buy
Product number Packaging Price Buy
216275-M 50mg $250 Buy
216275-M 216275-50MG $138 Buy
C4916 25mg $104 Buy
C2859 50mg $74 Buy
C2859 200mg $218 Buy

5-CARBOXYFLUORESCEIN DIACETATE Chemical Properties,Uses,Production

Uses

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein.

Uses

5-Carboxyfluorescein Diacetate is used in the synthesis of Rhodamines. Dyes and metabolites.

Biological Activity

Primary Target
Vital stain

552-30-7
79955-27-4
Synthesis of 5-CARBOXYFLUORESCEIN DIACETATE from Trimellitic Anhydride

5-CARBOXYFLUORESCEIN DIACETATE Preparation Products And Raw materials

Global( 167)Suppliers
Supplier Tel Email Country ProdList Advantage
Nanjing ChemLin Chemical Industry Co., Ltd.
025-83697070 product@chemlin.com.cn CHINA 3009 60
CONIER CHEM AND PHARMA LIMITED
+8618523575427 sales@conier.com China 49979 58
career henan chemical co
+86-0371-86658258 +8613203830695 factory@coreychem.com China 29792 58
SIMAGCHEM CORP
+86-5922680277 +86-13806087780 sale@simagchem.com China 17346 58
TargetMol Chemicals Inc.
+1-781-999-5354; +17819995354 marketing@targetmol.com United States 32466 58
BOC Sciences
16314854226; +16314854226 inquiry@bocsci.com United States 19853 58
Dayang Chem (Hangzhou) Co.,Ltd.
+86-0571-88938639 +8617705817739 info@dycnchem.com China 52705 58
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418684 +8618949823763 sales@tnjchem.com China 25356 58
Hebei Chuanghai Biotechnology Co., Ltd
+8615531151365 mina@chuanghaibio.com China 18126 58
TargetMol Chemicals Inc.
+1-781-999-5354; support@targetmol.com United States 39039 58

View Lastest Price from 5-CARBOXYFLUORESCEIN DIACETATE manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
5-CFDA pictures 2026-04-22 5-CFDA
79955-27-4
$33.00-47.00 98.39% 10g TargetMol Chemicals Inc.
5-CFDA pictures 2026-04-22 5-CFDA
79955-27-4
$33.00-47.00 98.39% 10g TargetMol Chemicals Inc.
5-diacetate FAM pictures 2025-06-07 5-diacetate FAM
79955-27-4
1g >95.00% 1g Xian confluore Biological Technology Co., Ltd.
  • 5-CFDA pictures
  • 5-CFDA
    79955-27-4
  • $33.00-47.00
  • 98.39%
  • TargetMol Chemicals Inc.
  • 5-CFDA pictures
  • 5-CFDA
    79955-27-4
  • $33.00-47.00
  • 98.39%
  • TargetMol Chemicals Inc.
  • 5-diacetate FAM pictures
  • 5-diacetate FAM
    79955-27-4
  • $0.00
  • >95.00%
  • Xian confluore Biological Technology Co., Ltd.

5-CARBOXYFLUORESCEIN DIACETATE Spectrum

5-Carboxy-di-O-acetylfluorescein, 5-CFDA 5-FAM DA 3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid 5-Carboxyfluorescein diacetate 3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid 5-CFDA [5-Carboxyfluorescein diacetate] 5-Carboxyfluorescein diacetate≥ 95% (HPLC) BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC) CARBOXYFLUORESCEIN DI-O-ACETATE 5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE 5(6)-FAM DIACETATE 5(6)-CARBOXY-DI-O-ACETYLFLUORESCEIN 5-carboxy' Uorescein Diacetate 5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem 5-Carboxyfluorescein Diacetate > Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- 5-diacetate FAM 5-CFDA,5CFDA,Inhibitor,inhibit,5 CFDA 5-CFDA (5-Carboxyfluorescein Diacetate), Fluorogenic non-selective esterase substrate 5-CFDA, 10 mM in DMSO 5(6)-Carboxyfluorescein diacetate, solution, DMSO solution 5-carboxydiacetic acidester CFDA 79955-27-4 2C25H16O9 Biochemicals and Reagents BioChemical Fluorescent Probes, Labels, Particles and Stains Detection marker Aromatics Heterocycles Intermediates & Fine Chemicals Pharmaceuticals Fluorescent Xanthene