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5-METHYLCYTOSINE

5-METHYLCYTOSINE structure

CAS No.: 554-01-8

Chemical Name:: 5-METHYLCYTOSINE

Synonyms: 4-Amino-5-methylpyrimidin-2(1H)-one;5-methylcytosin;5-METHYLCYTOSINE;Cytosine, 5-methyl-;5-Methylcytosine,>96%;5-Methylcytosine (~90%);5-Methylcytosine, 97%, 97%;5-METHYLCYTOSINE USP/EP/BP;4-Amino-5-methyl-2-pyrimidinol;5-Methylcytosine, 10 mM in DMSO

CBNumber:: CB5154906

Molecular Formula:: C5H7N3O

Molecular Weight:: 125.13

MDL Number:: MFCD00233537

MOL File:: 554-01-8.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-05-28 02:30:20

Product description	Number	Pack Size	Price
5-Methylcytosine ≥98%	38741	1g	$39
5-Methylcytosine ≥98%	38741	5g	$174
5-Methylcytosine ≥98%	38741	10g	$308
Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.	C9111	50ug	$403
Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.	C9111	250ug	$672
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5-METHYLCYTOSINE Properties

Melting point	270°C (rough estimate)
Boiling point	232.57°C (rough estimate)
Density	1.2602 (rough estimate)
refractive index	1.6510 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
solubility	Methanol (Sparingly, Heated)
pka	9.05±0.10(Predicted)
form	Crystalline
color	White to Off-White
Water Solubility	Soluble in water.
Merck	14,6050
InChI	InChI=1S/C5H7N3O/c1-3-2-7-5(9)8-4(3)6/h2H,1H3,(H3,6,7,8,9)
InChIKey	LRSASMSXMSNRBT-UHFFFAOYSA-N
SMILES	C1(=O)NC(N)=C(C)C=N1
CAS DataBase Reference	554-01-8(CAS DataBase Reference)
FDA UNII	6R795CQT4H

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H319-H317

Precautionary statements

P261-P272-P280-P302+P352-P333+P313-P321-P363-P501-P264-P280-P305+P351+P338-P337+P313P

RTECS

UW7362350

NFPA 704

	1
0		0

5-METHYLCYTOSINE price More Price(68)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	1g	$39	2026-04-30	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	5g	$174	2026-04-30	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	10g	$308	2026-04-30	Buy
Usbiological	C9111	Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.		50ug	$403	2026-06-03	Buy
Usbiological	C9111	Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.		250ug	$672	2026-06-03	Buy

Product number	Packaging	Price	Buy
38741	1g	$39	Buy
38741	5g	$174	Buy
38741	10g	$308	Buy
C9111	50ug	$403	Buy
C9111	250ug	$672	Buy

5-METHYLCYTOSINE Chemical Properties,Uses,Production

Chemical Properties

Crystals in prisms from water.

Uses

5-Methylcytosine is used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes. It is also employed in the production of 4-amino-5-hydroxy-5-methyl-2-oxo-2,5-dihydro-imidazole-1-carbaldehyde.

Definition

ChEBI: A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position.

IC 50

Microbial Metabolite; Human Endogenous Metabolite

Purification Methods

Crystallise it from water (solubility is 3.4%). The hydrochloride has m 299-301o (sintering at 280o) (from aqueous HCl/Me2CO). [Hitchings et al. J Biol Chem 177 537 1949, Cohn J Am Chem Soc 73 1539 1951, Beilstein 25 II 183, 25 III/IV 3727.]

5-METHYLCYTOSINE synthesis

Synthesis of 5-METHYLCYTOSINE from Thymine

5-METHYLCYTOSINE Preparation Products And Raw materials

Raw materials

5-METHYL-2-THIOCYTOSINE 5-methyl-4-(1,2,4-triazol-1-yl)-pyrimidin-2-(1H)-one 2-(Ethylthio)-5-methyl-4-pyrimidinamine 2(1H)-Pyrimidinone, 4-methoxy-5-methyl- (8CI,9CI) 4-chloro-2-ethylsulfanyl-5-methyl-pyrimidine

5-Methyl-2'-deoxycytidine 5-HydroxyMethyl cytidine 5-(Hydroxymethyl)-2'-deoxycytidine 5-HYDROXYMETHYLCYTOSINE (5-methyl)dithiouracil

5-formyl-2'-deoxycytidine 5-Methylcytidine Ethanol Thymine

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Preparation Products

1-[(2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione

5-METHYLCYTOSINE Suppliers

Global( 257)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Shanghai Zheyan Biotech Co., Ltd.	18017610038	zheyansh@163.com	CHINA	3619	58
career henan chemical co	+86-0371-86658258 +8613203830695	sales@coreychem.com	China	29812	58
Alchem Pharmtech,Inc.	8485655694	sales@alchempharmtech.com	United States	63687	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49979	58
Antai Fine Chemical Technology Co.,Limited	18503026267	info@antaichem.com	CHINA	9636	58
TargetMol Chemicals Inc.	+1-781-999-5354; +17819995354	marketing@targetmol.com	United States	32466	58
Hefei TNJ Chemical Industry Co.,Ltd.	+86-0551-65418671 +8618949823763	sales@tnjchem.com	China	34563	58
ANHUI WITOP BIOTECH CO., LTD	+8615255079626	eric@witopchemical.com	China	23541	58
Dideu Industries Group Limited	+86-86-15536356810 +8617392712697	1022@dideu.com	China	28760	58
AFINE CHEMICALS LIMITED	+86-571-85134551	info@afinechem.com	China	15081	58

Supplier	Advantage
Shanghai Zheyan Biotech Co., Ltd.	58
career henan chemical co	58
Alchem Pharmtech,Inc.	58
CONIER CHEM AND PHARMA LIMITED	58
Antai Fine Chemical Technology Co.,Limited	58
TargetMol Chemicals Inc.	58
Hefei TNJ Chemical Industry Co.,Ltd.	58
ANHUI WITOP BIOTECH CO., LTD	58
Dideu Industries Group Limited	58
AFINE CHEMICALS LIMITED	58

View Lastest Price from 5-METHYLCYTOSINE manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-05-11	5-Methylcytosine 554-01-8	$29.00		99.25%	10g	TargetMol Chemicals Inc.
2021-07-13	5-methylcytosine 554-01-8	$10.00-15.00	1KG	99%+ HPLC		Zhuozhou Wenxi import and Export Co., Ltd
2021-07-10	5-methylcytosine 554-01-8	$10.00-15.00	1KG	99%+ HPLC		Zhuozhou Wenxi import and Export Co., Ltd

5-Methylcytosine
554-01-8
$29.00
99.25%
TargetMol Chemicals Inc.

5-methylcytosine
554-01-8
$10.00-15.00
99%+ HPLC
Zhuozhou Wenxi import and Export Co., Ltd

5-methylcytosine
554-01-8
$10.00-15.00
99%+ HPLC
Zhuozhou Wenxi import and Export Co., Ltd

5-METHYLCYTOSINE Spectrum

5-METHYLCYTOSINE(554-01-8)¹HNMR

554-01-8(5-METHYLCYTOSINE)Related Search:

5'-O-(4,4'-DIMETHOXYTRITYL)-5-METHYL-N4-BENZOYL-2'-DEOXYCYTIDINE-3'-(2-CYANOETHYL-N,N-DIISOPROPYL)PHOSPHORAMIDITE 5-(TRIFLUOROMETHYL)-2'-DEOXYCYTIDINE 5-CARBOXYCYTOSINE 5-METHYLCYTOSINE 1-(2-Deoxy-beta-D-ribofuranosyl)-5-methylcytosine

2'-C-Methylcytidine 2-O-Methylcytosine 5-HYDROXYMETHYLCYTOSINE N4-Methylcytosine ,98%,N4-Methylcytosine Cytosine

5-Methylcytidine 5-METHYLCYTOSINE HYDROCHLORIDE FLUORESCEIN-12-DCTP BIOTIN-11-DDCTP 5'-O-(4,4'-DIMETHOXYTRITYL)-N4-BENZOYL-5-METHYL-2'-DEOXYCYTIDINE

5'-METHYL-2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE TRILITHIUM SALT N4-BENZOYL-5-METHYLDEOXYCYTIDINE 3-METHYLCYTOSINE HYDROCHLORIDE

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4-Amino-5-methyl-2-pyrimidinol Cytosine, 5-methyl- 5-Methylcytosine,>96% 2(1H)-Pyrimidinone, 4-amino-5-methyl- (9CI) 4-amino-5-methyl-3H-pyrimidin-2-one 5-METHYLCYTOSINE 2(1H)-Pyrimidinone, 6-amino-5-methyl- 2(1H)-Oxo-4(3H)-imino-5-methylpyrimidine 2(1H)-Oxo-4-amino-5-methylpyrimidine 2-Hydroxy-4-amino-5-methylpyrimidine 4-Amino-5-methyl-1,2-dihydropyrimidine-2-one 4-Amino-5-methylpyrimidin-2(1H)-one 4(6)-AMino-2-hydroxy-5-MethylpyriMidine 4-AMino-5-Methyl-2(1H)pyriMidinone 4-aMino-5-Methyl-1,2-dihydropyriMidin-2-one 5-methylcytosin 5-Methylcytosine, 97%, 97% 5-Methylcytosine (~90%) 5-METHYLCYTOSINE USP/EP/BP 5-Methylcytosine, 10 mM in DMSO 554-01-8 Nucleic acids Pyrimidines PYRIMIDINE Elisa Kit-Rat Elisa Kit