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4-Chloro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzofurazan structure

CAS No.: 10199-89-0

Chemical Name:: 4-Chloro-7-nitrobenzofurazan

Synonyms: NBD-CL;4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole;4-Chloro-7-nitro-1,2,3-benzoxadiazole;4-CHLORO-7-NITROBENZOFURAZAN;NBD CHLORIDE;NBD chloride,98%;4-chloro-7-nitrobenzofurazane;Benzofurazan, 4-chloro-7-nitro-;4-chloro-7-nitrobenzo-2,1,3-oxadiazole;7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE

CBNumber:: CB5173637

Molecular Formula:: C6H2ClN3O3

Molecular Weight:: 199.55

MDL Number:: MFCD00005808

MOL File:: 10199-89-0.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-05-28 02:01:11

Product description	Number	Pack Size	Price
4-Chloro-7-nitrobenzofurazan 98%	163260	1g	$56
4-Chloro-7-nitrobenzofurazan 98%	163260	5g	$78.3
NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T)	A5592	1g	$42
NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T)	A5592	5g	$121
4-Chloro-7-nitro-2,1,3-benzoxadiazole	TRC-C373895-5G	5g	$223
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4-Chloro-7-nitrobenzofurazan Properties

Melting point	97-99 °C(lit.)
Boiling point	333.1±45.0 °C(Predicted)
Density	2.0589 (rough estimate)
refractive index	1.5810 (estimate)
storage temp.	Inert atmosphere,Room Temperature
solubility	Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform.
form	Crystalline Powder
pka	-5.65±0.50(Predicted)
color	Yellow to light brown
Water Solubility	soluble
BRN	614212
InChI	InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
InChIKey	IGHBXJSNZCFXNK-UHFFFAOYSA-N
SMILES	N1=C2C([N+]([O-])=O)=CC=C(Cl)C2=NO1
CAS DataBase Reference	10199-89-0(CAS DataBase Reference)
EWG's Food Scores	1
FDA UNII	EQF2794IRE
EPA Substance Registry System	2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0)
UNSPSC Code	12352100
NACRES	NA.32

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H315-H319

Precautionary statements

P302+P352-P305+P351+P338

PPE

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Hazard Codes

Xn,Xi

Risk Statements

22-36/38

Safety Statements

36/37/39

RIDADR

UN 2811 6.1/PG 3

WGK Germany

RTECS

DF8002400

10-21

TSCA

TSCA listed

HS Code

29349990

Storage Class

11 - Combustible Solids

Hazard Classifications

Eye Irrit. 2
Skin Irrit. 2

NFPA 704

	1
2		0

4-Chloro-7-nitrobenzofurazan price More Price(76)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	163260	4-Chloro-7-nitrobenzofurazan 98%	10199-89-0	1g	$56	2026-04-30	Buy
Sigma-Aldrich	163260	4-Chloro-7-nitrobenzofurazan 98%	10199-89-0	5g	$78.3	2026-04-30	Buy
TCI Chemical	A5592	NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T)	10199-89-0	1g	$42	2026-04-30	Buy
TCI Chemical	A5592	NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling] >98.0%(T)	10199-89-0	5g	$121	2026-04-30	Buy
TRC	TRC-C373895-5G	4-Chloro-7-nitro-2,1,3-benzoxadiazole	10199-89-0	5g	$223	2026-06-03	Buy

Product number	Packaging	Price	Buy
163260	1g	$56	Buy
163260	5g	$78.3	Buy
A5592	1g	$42	Buy
A5592	5g	$121	Buy
TRC-C373895-5G	5g	$223	Buy

4-Chloro-7-nitrobenzofurazan Chemical Properties,Uses,Production

Chemical Properties

yellow to light brown crystalline powder

Uses

4-Chloro-7-nitrobenzofurazan is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. 4-Chloro-7-nitrobenzofurazan has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

Uses

4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide.

Definition

ChEBI: A benzoxadiazole that is 2,1,3-benzoxadiazole which is substituted at position 4 by chlorine and at position 7 by a nitro group.

General Description

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly sensitive chromogenic and fluorogenic reagent. It is reported to react spontaneously with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky′s diene), to afford regioselectively the silyl enol ether, via normal electron-demand Diels-Alder (NEDDA) reaction. A study of the molecular structure, vibrational spectra and NBO (Natural Bond Orbital) analysis of NBD–Cl has been undertaken. Its utility as a pre-column derivatization agent has been examined.

in vitro

4-Chloro-7-nitrobenzofurazan(NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides.

Purification Methods

Wash the solid with H2O, and it recrystallises from aqueous EtOH (1:1) as pale yellow needles. It sublimes in a vacuum [Gosh & Whitehouse Biochem J 108 155 1968, UV, NMR: Bolton et al. J Chem Soc 1004 1966].

4-Chloro-7-nitrobenzofurazan synthesis

Synthesis of 4-Chloro-7-nitrobenzofurazan from 4-CHLOROBENZOFURAZAN

4-Chloro-7-nitrobenzofurazan Preparation Products And Raw materials

Raw materials

Preparation Products

4-Nitro-7-piperidin-1-yl-2,1,3-benzoxadiazole 2-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)thio]ethanaMine

4-Chloro-7-nitrobenzofurazan Suppliers

Global( 269)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Hebei Chuanghai Biotechnology Co., Ltd	+86-15350571055	Sibel@chuanghaibio.com	China	8737	58
ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD	+86-15225627621 +86-13213167925	jiuyitime@fdachem.com	China	16505	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21533	55
ATK CHEMICAL COMPANY LIMITED	+undefined-21-51877795	ivan@atkchemical.com	China	32997	60
Hefei TNJ Chemical Industry Co.,Ltd.	+86-0551-65418679 +86-18949832763	info@tnjchem.com	China	2984	55
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29766	58
Alchem Pharmtech,Inc.	8485655694	sales@alchempharmtech.com	United States	63645	58
CONIER CHEM AND PHARMA LIMITED		sales@conier.com	China	49906	58
Fuxin Pharmaceutical	+86-021-021-50872116	contact@fuxinpharm.com	China	9976	58
TargetMol Chemicals Inc.	+1-781-999-5354; +1-00000000000	marketing@targetmol.com	United States	32431	58

Supplier	Advantage
Hebei Chuanghai Biotechnology Co., Ltd	58
ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD	58
Henan Tianfu Chemical Co.,Ltd.	55
ATK CHEMICAL COMPANY LIMITED	60
Hefei TNJ Chemical Industry Co.,Ltd.	55
career henan chemical co	58
Alchem Pharmtech,Inc.	58
CONIER CHEM AND PHARMA LIMITED	58
Fuxin Pharmaceutical	58
TargetMol Chemicals Inc.	58

View Lastest Price from 4-Chloro-7-nitrobenzofurazan manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-07-02	4-Chloro-7-nitrobenzofurazan 10199-89-0	$2.20-8.80	1KG	99%		ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD
2026-03-20	4-Chloro-7-nitrobenzofurazan 10199-89-0	$10.00	1KG	99%	10 mt	Hebei Chuanghai Biotechnology Co., Ltd
2024-05-31	4-Chloro-7-nitro-1,2,3-benzoxadiazole 10199-89-0	$1150.00	1g	98	500 Kg	R&D Scientific Inc.

4-Chloro-7-nitrobenzofurazan
10199-89-0
$2.20-8.80
99%
ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD

4-Chloro-7-nitrobenzofurazan
10199-89-0
$10.00
99%
Hebei Chuanghai Biotechnology Co., Ltd

4-Chloro-7-nitro-1,2,3-benzoxadiazole
10199-89-0
$1150.00
98
R&D Scientific Inc.

4-Chloro-7-nitrobenzofurazan Spectrum

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)MS 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)¹HNMR 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)IR1 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)IR2 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole(10199-89-0)Raman

10199-89-0(4-Chloro-7-nitrobenzofurazan)Related Search:

4-Chloro-7-nitrobenzofurazan Nitrobenzene BENZOFURAZAN N-BENZYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 7-CHLORO-4-NITRO-5-PYRROLIDIN-1-YL-BENZO[1,2,5]OXADIAZOLE

7-CHLORO-4-NITRO-5-PIPERIDINO-2,1,3-BENZOXADIAZOLE 5-AMINO-7-CHLORO-4-NITROBENZOFURAZAN 7-CHLORO-N,N-DIETHYL-4-NITRO-2,1,3-BENZOXADIAZOL-5-AMINE 2-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-ETHANOL 3-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PROPAN-1-OL

AKOS BB-7022 7-CHLORO-5-(4-METHYL-PIPERAZIN-1-YL)-4-NITRO-BENZO[1,2,5]OXADIAZOLE 7-CHLORO-5-MORPHOLIN-4-YL-4-NITRO-BENZO[1,2,5]OXADIAZOLE (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-PYRIDIN-2-YL-AMINE N-[4-(7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YLAMINO)-PHENYL]-ACETAMIDE

TIMTEC-BB SBB005650 (7-CHLORO-4-NITRO-BENZO[1,2,5]OXADIAZOL-5-YL)-DIMETHYL-AMINE 4-CHLORO-5,7-DINITROBENZOFURAZAN

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4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE 7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- 4-chloro-7-nitrobenzo-2,1,3-oxadiazole NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling] 4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride NBC-1 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) 4-Chloro-7-nitro-1,2 7-Chloro-4-nitro-2,1,3-benzoxadiazole NSC 228140 4-Chloro-7-nitrobenzofurazan 4-Chloro-7-nitro-2,1,3-benzoxadiazole 4-Chloro-7-nitro-2,1,3-benzoxadiazole 99% 4-chloro-7-nitro-benzofuraza 4-nitro-7-chlorobenzofurazan nbd-c1 7-CHLORO-4-NITRO-2,1,3-BENZOXADIAZOL 7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE 7-CHLORO-4-NITROBENZOFURAZAN AKOS BC-0686 4-CHLORO-7-NITRO-2,1,3-BENZOXADIAZOLE 4-CHLORO-7-NITROBENZ-2-OXA-1,3-DIAZOLE 4-CHLORO-7-NITRO-BENZO[1,2,5]OXADIAZOLE 4-CHLORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE 4-CHLORO-7-NITROBENZOFURAZAN 4-CHLORO-7-NITROBENZUFURAZAN 4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl ) 4-Chloro-7-nitrobenzo-2-furazan, NBF-Cl 1-Chloro-4-nitrobenzoxadiazole LABOTEST-BB LT00238516 NBD CHLORIDE TIMTEC-BB SBB005345 NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* 7-CHLORO-4-NITROBENZ-2-OXA-1,3-DIAZOLE Shokat-update-comp17 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole)[for HPLC Labeling]> NBD-Cl [4-Chloro-7-nitrobenzofurazan oro-7-nitrobenzo-2-oxa-1,3-diazoL 4-Chloro-7-nitro-2,1,3-benzoxadiazole(NBD-Cl) 4-Choro-7-nitrobenzofurazan 4-Chloro-7-nitrobenzo-2-furazan 4-Chloro-7-nitrobenzo-2-oxa-1,3-oxadiazole NBD-Cl, Amine-reactive fluorogenic dye NBD-Cl, 10 mM in DMSO 4-Chloro-7-nitro-1,2,3-benzoxadiazole 4-chloro-7-nitrobenzo[c][1,2,5]oxadiazole 4-chloro-7-nitrobenzofurazane Benzofurazan, 4-chloro-7-nitro- NBD chloride,98% NBD-CL 10199-89-0 C6H2ClN3O3 Mercapto Group Labeling Reagents for Fluorescence HPLC HPLC Labeling Reagents Fluorescence Detection (HPLC Labeling Reagents) Amino Group Labeling Reagents for Fluorescence HPLC Functional Group Reactive Labels