5(6)-Carboxyfluorescein
- CAS No.
- 72088-94-9
- Chemical Name:
- 5(6)-Carboxyfluorescein
- Synonyms
- 5(6)-FAM;DCFA;5(6)-Carboxyfluorescein - mixture;5(6)-Carboxyfluoresc;5(6)-CARBOXYLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;5(6)-CARBOXYFLUORESCEIN;5(6)-Carboxyfluoresceine];4(5)-Carboxyfluoresceine];Fluoresceincarboxylic Acid
- CBNumber:
- CB5257173
- Molecular Formula:
- C42H24O14
- Molecular Weight:
- 752.63
- MDL Number:
- MFCD00151081
- MOL File:
- 72088-94-9.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 275 °C |
|---|---|
| Boiling point | 464.9°C (rough estimate) |
| Density | 1.3418 (rough estimate) |
| refractive index | 1.6380 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
| form | Solid |
| color | Light Yellow to Dark Brown |
| Water Solubility | slightly soluble |
| λmax | 492 nm |
| ε(extinction coefficient) |
12134 at 284nm in 0.1 M NaOH at 0.005g/L 43060 at 239nm in 0.1 M NaOH at 0.005g/L |
| Major Application |
diagnostic assay manufacturing hematology histology |
| InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
| SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
| UNSPSC Code | 12352005 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H319 | |||||||||
| Precautionary statements | P305+P351+P338 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Hazard Codes | Xi,Xn | |||||||||
| Risk Statements | 36-36/37/38-20/21/22 | |||||||||
| Safety Statements | 37/39-26-36-22 | |||||||||
| WGK Germany | 3 | |||||||||
| TSCA | TSCA listed | |||||||||
| HS Code | 29322090 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| NFPA 704 |
|
5(6)-Carboxyfluorescein price More Price(82)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 8.51082 | 5(6)-Carboxyfluorescein Novabiochem? | 72088-94-9 | 1g | $63.5 | 2026-04-30 | Buy |
| Sigma-Aldrich | 8.51082 | 5(6)-Carboxyfluorescein Novabiochem? | 72088-94-9 | 5G | $212 | 2026-04-30 | Buy |
| Sigma-Aldrich | 21877 | 5(6)-Carboxyfluorescein BioReagent, suitable for fluorescence, ≥95% (HPLC) | 72088-94-9 | 1g | $67.9 | 2026-04-30 | Buy |
| Sigma-Aldrich | 21877 | 5(6)-Carboxyfluorescein BioReagent, suitable for fluorescence, ≥95% (HPLC) | 72088-94-9 | 5G | $230 | 2026-04-30 | Buy |
| Cayman Chemical | 17172 | 5(6)-Carboxyfluorescein ≥90%(mixtureofisomers) | 72088-94-9 | 1g | $38 | 2026-04-30 | Buy |
5(6)-Carboxyfluorescein Chemical Properties, Uses, Production
Description
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
Chemical Properties
Dark-Orange to Brown Solid
Uses
A popular probe of determination of intracellular pH.
Uses
5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
Uses
As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
Definition
ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.
reaction suitability
reagent type: fluorescent-labelling reagent
Solubility in organics
5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.
5(6)-Carboxyfluorescein Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Okeanos Tech Co,. Ltd. | 010-62651721 18610486005 | minghui.yu@okeanos.com.cn | China | 495 | 60 |
| Nanjing Habo Medical Technology Co., Ltd. | 13376080562 13376090521 | sales@habotech.com | China | 724 | 60 |
| changzhou ansciep chemical co., ltd. | 0519-86305871 13961213398; | tina@ansciepchem.com | China | 1600 | 58 |
| Shanghai Boyle Chemical Co., Ltd. | sales@boylechem.com | China | 2915 | 55 | |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| Alfa Aesar | 400-6106006 | saleschina@alfa-asia.com | China | 30103 | 84 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Beijing Ouhe Technology Co., Ltd | 13552068683 13522913783 | 2355560935@qq.com | China | 11777 | 60 |
| Nanjing Search Biotech Co., Ltd | +86-025-81682922 +86-13515111588 | sales@searchbio.com.cn | China | 137 | 68 |
View Latest Price from 5(6)-Carboxyfluorescein manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-02 | 5(6)-Carboxyfluorescein
72088-94-9
|
$1.20-35.00 | 1kg | 99% | Henan Fengda Chemical Co., Ltd | ||
 |
2026-07-15 | 5(6)-Carboxyfluorescein
72088-94-9
|
$29.00 | 98.65% | 10g | TargetMol Chemicals Inc. | ||
 |
2025-06-27 | 5(6)-Carboxyfluorescein
72088-94-9
|
1KG | 99% | 500000kg | Hebei Chuanghai Biotechnology Co., Ltd |
-
- 5(6)-Carboxyfluorescein
72088-94-9
- $1.20-35.00
- 99%
- Henan Fengda Chemical Co., Ltd
-
- 5(6)-Carboxyfluorescein
72088-94-9
- $29.00
- 98.65%
- TargetMol Chemicals Inc.
-
- 5(6)-Carboxyfluorescein
72088-94-9
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
