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BCIP/NBT

BCIP/NBT Structure
BCIP/NBT structure
CAS No.
Chemical Name:
BCIP/NBT
Synonyms
NBT/BCIP;BCIP/NBT;BCIP/INT;BM PURPLE;BCIP/NBT PLUS;BCIP/NBT-BLUE;NBT-X-PHOSPHATE;SIGMAFAST? BCIP?/NBT;NBT-BCIP(R) solution;INT/BCIP TOLUIDINE SALT
CBNumber:
CB5448175
Molecular Formula:
C48H34BrClN11O10P
Molecular Weight:
1071.18
MDL Number:
MFCD00466954
MOL File:
Mol file
TDS File:
TDS
Last updated:2026-05-28 04:47:53
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BCIP/NBT Properties

Flash point 67 °C
storage temp. -20°C
form tablet
color dark purple
Water Solubility water: 10mL, clear, yellow
UNSPSC Code 12352204
NACRES NA.83

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P261-P305+P351+P338
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26
WGK Germany  2
Storage Class 12 - Non Combustible Liquids
Hazard Classifications Skin Sens. 1

BCIP/NBT price More Price(6)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 203790 BCIP/NBT 100 mL $146 2026-04-30 Buy
Sigma-Aldrich B1911 BCIP®/NBT Liquid Substrate System ready to use solution 100 mL $111 2025-07-31 Buy
Sigma-Aldrich B1911 BCIP®/NBT Liquid Substrate System ready to use solution 100mL $108 2024-03-01 Buy
Sigma-Aldrich B5655 SIGMAFAST™ BCIP®/NBT tablet 5TABLETS $58.3 2023-06-20 Buy
Sigma-Aldrich B5655 SIGMAFAST™ BCIP®/NBT tablet 25TABLETS $204 2023-06-20 Buy
Product number Packaging Price Buy
203790 100 mL $146 Buy
B1911 100 mL $111 Buy
B1911 100mL $108 Buy
B5655 5TABLETS $58.3 Buy
B5655 25TABLETS $204 Buy

BCIP/NBT Chemical Properties,Uses,Production

Uses

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

Uses

SIGMAFAST BCIP/NBT has been used as a substrate

  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

General Description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

BCIP/NBT Preparation Products And Raw materials

Raw materials

Preparation Products

BCIP/NBT Suppliers

Global( 40)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51389 80
Nantong Feiyu Biological Technology CO.,LTD 0513-68819626 15152877458 feiyubio@163.com China 4807 58
Shanghai Yaji Biological Technology Co., Ltd. 021-34661275 15301693058 yajikit@163.com China 9925 58
Shanghai Aladdin Biochemical Technology Co.,Ltd. 6206333 13167063860 anhua.mao@aladdin-e.com China 43293 58
Supplier Advantage
Sigma-Aldrich 80
Nantong Feiyu Biological Technology CO.,LTD 58
Shanghai Yaji Biological Technology Co., Ltd. 58
Shanghai Aladdin Biochemical Technology Co.,Ltd. 58

(BCIP/NBT)Related Search:

Uracil DNA Glycosylase (UDG) dNTP Mix 25mM each STREPTAVIDIN-HRP TTH DNA POLYMERASE Hotstart HiTaq Two-Step RT-PCR Mix
Hotstart Master PCR Mix (25X) Hotstart HiTaq PCR Mix (2X) dN(U)TP Mix 20/40mM each Hotstart HiTaq DNA Polymerase Taq Antibody (10U/ul)
Abstart Master PCR Mix (25X) Tth One-Step RT-PCR Mix Hotstart HiTaq One-Step RT-PCR Mix AP-Streptavidin Platinum Taq DNA Polymerase
Abstart PCR Mix (2X) Hotstart HiTaq One-Step RT-PCR Mix (Heat-labile UDG)

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BCIP/NBT 1-STEP NBT/BCIP SOLUTION 5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE/IODONITROTETRAZOLIUM CHLORIDE 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/P-IODONITROTETRAZOLIUM 5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/IODONITROTETRAZOLIUM BCIP/NBT LIQUID SUBSTRATE SYSTEM BCIP/NBT PLUS BCIP/NBT STABILIZED CHROMOGEN NITRO-BLUE TETRAZOLIUM CHLORIDE, 5-BROMO-4-CHLORO-INDOLYLPHOSPHATE NBT/BCIP NBT/BCIP PLUS SUPPRESSOR NBT-BCIP(R) solution SIGMAFAST? BCIP?/NBT 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT 5-BROMO-4 CHLORO-3-INDOLYL-PHOSPHATE AND NITROBLUE TETRAZOLIUM 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/NITROBLUE TETRAZOLIUM 5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/NITROBLUETETRAZOLIUM INT/BCIP TOLUIDINE SALT BCIP/INT BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE BCIP/NBT-BLUE BM PURPLE NITRO BLUE TETRA-ZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT NBT/BCIP SUBSTRATE SOLN NBT/BCIP TOLUIDINE SALT NBT/BCIP WITH LEVAMISOL NBT-X-PHOSPHATE BCIP(R)/NBT, NBT-X-phosphate