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CATALASE ASSAY KIT

CAS No.
Chemical Name:
CATALASE ASSAY KIT
Synonyms
CATALASE ASSAY KIT
CBNumber:
CB6509833
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2026-01-13 11:23:37

CATALASE ASSAY KIT Properties

storage temp. 2-8°C
Specific Activity 0.25 - 4nmol/min⋅mL
Major Application pharmaceutical
UNSPSC Code 41116133
NACRES NA.84

SAFETY

Risk and Safety Statements

WGK Germany  WGK 2
Storage Class 10 - Combustible liquids

CATALASE ASSAY KIT price More Price(3)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 219265 Catalase Assay Kit 1kit $902 2026-04-30 Buy
Cayman Chemical 707002 Catalase Assay Kit 96Well $402 2026-04-30 Buy
Cayman Chemical 707002 Catalase Assay Kit 480Well $803 2026-04-30 Buy
Product number Packaging Price Buy
219265 1kit $902 Buy
707002 96Well $402 Buy
707002 480Well $803 Buy

CATALASE ASSAY KIT Chemical Properties, Uses, Production

Description

Catalase (EC 1.11.1.6; 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS production. This enzyme catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While the aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. In humans, the highest levels of catalase are found in liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. The Cayman Chemical Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured spectrophotometrically with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald) as the chromogen. Purpald specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color. The assay can be used to measure CAT activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.

References

[1] LARS H. JOHANSSON  L. A H B. A spectrophotometric method for determination of catalase activity in small tissue samples[J]. Analytical biochemistry, 1988, 174 1: Pages 331-336. DOI: 10.1016/0003-2697(88)90554-4
[2] CONRAD R. WHEELER. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity[J]. Analytical biochemistry, 1990, 184 2: Pages 193-199. DOI: 10.1016/0003-2697(90)90668-y
[3] CONG CONG. Sinigrin attenuates angiotensin IIinduced kidney injury by inactivating nuclear factorκB and extracellular signalregulated kinase signaling in vivo and in vitro.[J]. International journal of molecular medicine, 2021, 48 2. DOI: 10.3892/ijmm.2021.4994
[4] HAMID ARAZI  Abbas A  Heidar Mohammadjafari. Use of anabolic androgenic steroids produces greater oxidative stress responses to resistance exercise in strength-trained men[J]. Toxicology Reports, 2017, 4: Pages 282-286. DOI: 10.1016/j.toxrep.2017.05.005
[5] KAMAL DATTA. Exposure to heavy ion radiation induces persistent oxidative stress in mouse intestine.[J]. PLoS ONE, 2012: e42224. DOI: 10.1371/journal.pone.0042224

CATALASE ASSAY KIT Preparation Products And Raw materials

Raw materials

Preparation Products

CATALASE ASSAY KIT Suppliers

Global Suppliers ( 22)
Supplier Tel Email Country ProdList Advantage
Shanghai YuanYe Biotechnology Co., Ltd. 15026964105 2881489226@qq.com China 89667 60
amyjet 4006800868;027-59626688 18771149750 sales@amyjet.com China 2909 58
Beijing Boorsen Biotechnology Co., Ltd 400-901-9800 18611424007 cis@bioss.com.cn China 9185 58
CATALASE ASSAY KIT