Thioflavin T
- CAS No.
- 2390-54-7
- Chemical Name:
- Thioflavin T
- Synonyms
- THIOFLAVIN T;THIOFLAVINE T;BASIC YELLOW 1;CI 49005;2-[4-(dimethylamino)phenyl]-3,6-dimethyl-benzothiazoliuchloride;ThiofL;avin T;CI NO 49005;hioflavineT;Setoflavin T
- CBNumber:
- CB6676791
- Molecular Formula:
- C17H19ClN2S
- Molecular Weight:
- 318.86
- MDL Number:
- MFCD00011944
- MOL File:
- 2390-54-7.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 211-212 °C (decomp) |
|---|---|
| Density | 1.301g/cm3 at 20℃ |
| vapor pressure | 0Pa at 25℃ |
| storage temp. | Store below +30°C. |
| solubility | methanol: soluble1mg/mL |
| Colour Index | 49005 |
| form | Powder |
| color | Yellow to ochre-yellow |
| Water Solubility | water: 5mg/mL |
| BRN | 3922452 |
| InChI | InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1 |
| InChIKey | JADVWWSKYZXRGX-UHFFFAOYSA-M |
| SMILES | C1(=[N+](C)C2C=CC(C)=CC=2S1)C1C=CC(N(C)C)=CC=1.[Cl-] |
| LogP | 3.02 at 25℃ and pH7.98 |
| CAS DataBase Reference | 2390-54-7(CAS DataBase Reference) |
| FDA UNII | CAX9SG0II0 |
| EPA Substance Registry System | Benzothiazolium, 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-, chloride (2390-54-7) |
| UNSPSC Code | 12171501 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
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|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H302-H315-H319-H332-H335 | |||||||||
| Precautionary statements | P261-P280-P305+P351+P338 | |||||||||
| Safety Statements | 24/25 | |||||||||
| RIDADR | 2811 | |||||||||
| WGK Germany | 3 | |||||||||
| TSCA | TSCA listed | |||||||||
| HazardClass | 6.1(b) | |||||||||
| PackingGroup | III | |||||||||
| HS Code | 32041300 | |||||||||
| Storage Class | 6.1C - Combustible acute toxic Cat.3 toxic compounds or compounds which causing chronic effects |
|||||||||
| Hazard Classifications | Acute Tox. 3 Oral Aquatic Acute 1 Aquatic Chronic 1 Eye Dam. 1 Skin Sens. 1 |
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| REACH Registrations | Active | |||||||||
| NFPA 704 |
|
Thioflavin T price More Price(59)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | T3516 | Thioflavin T used as stain for amyloid | 2390-54-7 | 5g | $39.5 | 2026-04-30 | Buy |
| Sigma-Aldrich | 596200 | Thioflavin T | 2390-54-7 | 500mg | $142 | 2026-04-30 | Buy |
| Sigma-Aldrich | 8.40072 | Thioflavine T (C.I.No.49005) ≥73% sulfur basis | 2390-54-7 | 5G | $89.7 | 2025-07-31 | Buy |
| TCI Chemical | T0558 | Basic Yellow 1 | 2390-54-7 | 25g | $47 | 2026-04-30 | Buy |
| Cayman Chemical | 32553 | Thioflavin T ≥90% | 2390-54-7 | 10g | $44 | 2026-04-30 | Buy |
Thioflavin T Chemical Properties,Uses,Production
Chemical Properties
yellow to ochre-yellow powder
Physical properties
In its free state, Thioflavin T (ThT) exhibits a maximum absorption wavelength of approximately 385 nm (near the UV-visible boundary) and a maximum emission wavelength of approximately 445 nm. Upon binding to amyloid fibrils, these spectral peaks undergo a red shift; the maximum excitation peak of the bound state is around 440–450 nm, and the maximum emission peak is around 480–490 nm.
History
In 1959, Vassar and Culling first employed Thioflavin T (ThT) for the fluorescence imaging of amyloid plaques, revealing its potential for histological staining. In 1993, LeVine standardized the method for quantifying fibril formation using ThT, providing a simple fluorescence-based approach for studying protein folding and aggregation kinetics[1].
Uses
Thioflavin T (ThT) is usually used for detecting several types systemic amyloid proteins, including Alzheimer's disease, prion diseases. Currently, the ThT fluorescence regulation strategies mainly include amyloid fibrils binding, DNA modification, metal ion coordination, micelle regulation, etc. ThT is also gradually used in the detection of heavy metal ions, biotoxins, and residues of pesticide and veterinary drug, due to its cheap price, good organism compatibility, light stability, and sensitivity to the environment[2].
Uses
Textile dyeing, fluorescent sign paints, in combination with green or blue pigments to produce brilliant greens, phosphotungstic pigments.
Uses
A dye used to visualize beta-amyloid plaques, like those associated with Alzheimer's Disease
Definition
ChEBI: Thioflavine T is an organic chloride salt having 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium as the counterion. It is widely used to visualise and quantify the presence of amyloids, both in vitro and in vivo. It has a role as a fluorochrome, a histological dye and a geroprotector. It contains a thioflavin T cation.
Preparation
4-(6-Methyl-2,3-dihydrobenzo[d]thiazol-2-yl)benzenaminewith methanol and hydrochloric acid or sulfuric acid, at 160 ~ 170 ℃ methylation under pressure.
Application
Upon binding to amyloid fibrils, Thioflavin T (ThT) exhibits a significant increase in fluorescence intensity, making it a common tool in research on protein aggregation-related diseases such as Alzheimer's disease and Parkinson's disease. The figure below shows the result of ThT staining[3].
General Description
Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid. Thioflavin T has been used in histology and for protein characterization.
Biochem/physiol Actions
Cell permeable: yes
storage
Store at -20°C
Properties and Applications
colourful green light yellow. Soluble in cold water, soluble in hot water for yellow, easily soluble in ethanol and yellow, with green fluorescent. The strong sulfuric acid to colorless, a green fluorescent, diluted into light yellow.
| Standard( Silk ) | Light Fastness | Persperation Fastness | Ironing Fastness | Soaping | |||
| Fading | Stain | Fading | Stain | Fading | Stain | ||
| C | 3 | 2-3 | 4 | 1 | |||
Purification Methods
Crystallise the chloride from *benzene/EtOH (1:1). [Beilstein 27 III/IV 5052.]
References
[1] LeVine, H. (1993). Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution. Protein Science, 2 3, 404–410. https://doi.org/10.1002/pro.5560020312
[2] Precupas, A., & Matei, I. (2025). Thioflavin T: induced circular dichroism and density functional theory as promising approach in amyloid fibril assays. Applied Spectroscopy Reviews, 61(1), 78–116. https://doi.org/10.1080/05704928.2025.2501692
[3] Orchard, G. E. (2023). Gout with Associated Cutaneous AA Amyloidosis: A case Report and Review of the literature. British Journal of Biomedical Science, 80, 11442. https://doi.org/10.3389/bjbs.2023.11442
Thioflavin T Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Tangshan Moneide Trading Co., Ltd. | 0315-8309571 15633399667 | sales@moneidechem.com | China | 703 | 57 |
| BIOISCO(Lianyungang, Jiangsu) Biotechnology Co., Ltd | 18082049515 | 2757382053@qq.com | China | 2995 | 58 |
| Hubei Shiteng Chemical Technology Co. LTD | 18971850150 19086915685 | 3053385855@qq.com | China | 5000 | 58 |
| Shanghai Biological Technology Development Co., Ltd. | 021-69955236-807 13918189704 | chinaruji@chinaruji.com | China | 5093 | 55 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Beijing Ouhe Technology Co., Ltd | 13552068683 13522913783 | 2355560935@qq.com | China | 11777 | 60 |
| Nanjing Chemlin Chemical Co., Ltd | 025-83697070 13770331960 | info@chemlin.com.cn | China | 16305 | 64 |
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- Thioflavin T (ThT) is a commonly used probe to monitor vitro amyloid fibril formation.
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View Lastest Price from Thioflavin T manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-07-21 | THIOFLAVIN T
2390-54-7
|
1kg | 99% | 20tons | Hubei Lidu New Material Technology Co., Ltd | ||
 |
2026-07-16 | Thioflavin T
2390-54-7
|
$29.00 | 98.51% | 10g | TargetMol Chemicals Inc. | ||
 |
2026-03-20 | Thioflavine T
2390-54-7
|
$1.00 | 1KG | 99% | 10 mt | Hebei Chuanghai Biotechnology Co., Ltd |
-
- THIOFLAVIN T
2390-54-7
- 99%
- Hubei Lidu New Material Technology Co., Ltd
-
- Thioflavin T
2390-54-7
- $29.00
- 98.51%
- TargetMol Chemicals Inc.
-
- Thioflavine T
2390-54-7
- $1.00
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
