- THIOFLAVIN T
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-
2026-07-21
- CAS:2390-54-7
- Min. Order: 1kg
- Purity: 99%
- Supply Ability: 20tons
- Thioflavin T
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- $29.00
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2026-07-16
- CAS:2390-54-7
- Purity: 98.51%
- Supply Ability: 10g
- Thioflavine T
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- $1.00
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2026-03-20
- CAS:2390-54-7
- Min. Order: 1KG
- Purity: 99%
- Supply Ability: 10 mt
Related articles - The Thioflavin T assay
- Thioflavin T (ThT) is a commonly used probe to monitor vitro amyloid fibril formation.
- Oct 31，2024
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| | Thioflavin T Basic information |
| Product Name: | Thioflavin T | | Synonyms: | Benzothiazolium,2-[4-(dimethylamino)phenyl]-3,6-dimethyl-,chloride;Thioflavine T (C.I.No.49005) for synthesis;Thioflavin T (Absorbance(E1%1cm) >=750 (410 nm to 414 nm, H2O);Thioflavin T *CAS 2390-54-7*;avin T;ThiofL;LABOTEST-BB LT00451909;CI NO 49005 | | CAS: | 2390-54-7 | | MF: | C17H19ClN2S | | MW: | 318.86 | | EINECS: | 219-228-9 | | Product Categories: | Biochemistry;Vitamin Derivatives;Vitamins | | Mol File: | 2390-54-7.mol |  |
| | Thioflavin T Chemical Properties |
| Melting point | 211-212 °C (decomp) | | density | 1.301g/cm3 at 20℃ | | vapor pressure | 0Pa at 25℃ | | storage temp. | Store below +30°C. | | solubility | methanol: soluble1mg/mL | | Colour Index | 49005 | | form | Powder | | color | Yellow to ochre-yellow | | Water Solubility | water: 5mg/mL | | BRN | 3922452 | | InChI | InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1 | | InChIKey | JADVWWSKYZXRGX-UHFFFAOYSA-M | | SMILES | C1(=[N+](C)C2C=CC(C)=CC=2S1)C1C=CC(N(C)C)=CC=1.[Cl-] | | LogP | 3.02 at 25℃ and pH7.98 | | CAS DataBase Reference | 2390-54-7(CAS DataBase Reference) | | EPA Substance Registry System | Benzothiazolium, 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-, chloride (2390-54-7) |
| Safety Statements | 24/25 | | RIDADR | 2811 | | WGK Germany | 3 | | TSCA | TSCA listed | | HazardClass | 6.1(b) | | PackingGroup | III | | HS Code | 32041300 | | Storage Class | 6.1C - Combustible acute toxic Cat.3
toxic compounds or compounds which causing chronic effects | | Hazard Classifications | Acute Tox. 3 Oral
Aquatic Acute 1
Aquatic Chronic 1
Eye Dam. 1
Skin Sens. 1 |
| | Thioflavin T Usage And Synthesis |
| Chemical Properties | yellow to ochre-yellow powder | | Physical properties | In its free state, Thioflavin T (ThT) exhibits a maximum absorption wavelength of approximately 385 nm (near the UV-visible boundary) and a maximum emission wavelength of approximately 445 nm. Upon binding to amyloid fibrils, these spectral peaks undergo a red shift; the maximum excitation peak of the bound state is around 440–450 nm, and the maximum emission peak is around 480–490 nm. | | History | In 1959, Vassar and Culling first employed Thioflavin T (ThT) for the fluorescence imaging of amyloid plaques, revealing its potential for histological staining. In 1993, LeVine standardized the method for quantifying fibril formation using ThT, providing a simple fluorescence-based approach for studying protein folding and aggregation kinetics[1]. | | Uses | Thioflavin T (ThT) is usually used for detecting several types systemic amyloid proteins, including Alzheimer's disease, prion diseases. Currently, the ThT fluorescence regulation strategies mainly include amyloid fibrils binding, DNA modification, metal ion coordination, micelle regulation, etc. ThT is also gradually used in the detection of heavy metal ions, biotoxins, and residues of pesticide and veterinary drug, due to its cheap price, good organism compatibility, light stability, and sensitivity to the environment[2]. | | Uses | Textile dyeing, fluorescent sign paints, in combination
with green or blue pigments to produce
brilliant greens, phosphotungstic pigments. | | Uses | A dye used to visualize beta-amyloid plaques, like those associated with Alzheimer's Disease | | Definition | ChEBI: Thioflavine T is an organic chloride salt having 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium as the counterion. It is widely used to visualise and quantify the presence of amyloids, both in vitro and in vivo. It has a role as a fluorochrome, a histological dye and a geroprotector. It contains a thioflavin T cation. | | Preparation | 4-(6-Methyl-2,3-dihydrobenzo[d]thiazol-2-yl)benzenaminewith methanol and hydrochloric acid or sulfuric acid, at 160 ~ 170 ℃ methylation under pressure. | | Application | Upon binding to amyloid fibrils, Thioflavin T (ThT) exhibits a significant increase in fluorescence intensity, making it a common tool in research on protein aggregation-related diseases such as Alzheimer's disease and Parkinson's disease. The figure below shows the result of ThT staining[3].
 | | General Description | Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid. Thioflavin T has been used in histology and for protein characterization. | | Biochem/physiol Actions | Cell permeable: yes | | storage | Store at -20°C | | Properties and Applications | colourful green light yellow. Soluble in cold water, soluble in hot water for yellow, easily soluble in ethanol and yellow, with green fluorescent. The strong sulfuric acid to colorless, a green fluorescent, diluted into light yellow.
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Standard( Silk )
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Light Fastness
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Soaping
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3
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2-3
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1
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| | Purification Methods | Crystallise the chloride from *benzene/EtOH (1:1). [Beilstein 27 III/IV 5052.] | | References |
[1] LeVine, H. (1993). Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution. Protein Science, 2 3, 404–410. https://doi.org/10.1002/pro.5560020312
[2] Precupas, A., & Matei, I. (2025). Thioflavin T: induced circular dichroism and density functional theory as promising approach in amyloid fibril assays. Applied Spectroscopy Reviews, 61(1), 78–116. https://doi.org/10.1080/05704928.2025.2501692
[3] Orchard, G. E. (2023). Gout with Associated Cutaneous AA Amyloidosis: A case Report and Review of the literature. British Journal of Biomedical Science, 80, 11442. https://doi.org/10.3389/bjbs.2023.11442
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| | Thioflavin T Preparation Products And Raw materials |
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