Trifluridine
- CAS No.
- 70-00-8
- Chemical Name:
- Trifluridine
- Synonyms
- TFT;TRIFLUOROTHYMIDINE;viroptic;f3t;FTD;nsc75520;Triflurdine;Triflurdine (Viroptic);Thymidine, α,α,α-trifluoro-;2,4(1h,3h)-pyrimidinedione,1-(2-deoxy-beta-d-ribofuranosyl)-5-(trifluorometh
- CBNumber:
- CB6768812
- Molecular Formula:
- C10H11F3N2O5
- Molecular Weight:
- 296.2
- MDL Number:
- MFCD00006534
- MOL File:
- 70-00-8.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 190-193 °C (lit.) |
|---|---|
| Density | 1.4365 (estimate) |
| refractive index | 50 ° (C=1, H2O) |
| storage temp. | 2-8°C |
| solubility | Soluble in DMSO (up to 25 mg/ml) or in Water (up to 14 mg/ml) |
| pka | pKa 7.85 (Uncertain) |
| form | solid |
| color | Off-white |
| biological source | synthetic |
| Water Solubility | water: 50mg/mL, clear, colorless |
| Merck | 14,9687 |
| Henry's Law Constant | 1.0×1011 mol/(m3Pa) at 25℃, HSDB (2015) |
| Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or distilled water may be stored at -20°C for up to 1 month. |
| InChI | InChI=1S/C10H11F3N2O5/c11-10(12,13)4-2-15(9(19)14-8(4)18)7-1-5(17)6(3-16)20-7/h2,5-7,16-17H,1,3H2,(H,14,18,19)/t5-,6-,7-/m1/s1 |
| InChIKey | VSQQQLOSPVPRAZ-FSDSQADBSA-N |
| SMILES | [C@H]1(O)[C@@H](CO)O[C@@H](N2C=C(C(F)(F)F)C(=O)NC2=O)C1 |
| CAS DataBase Reference | 70-00-8(CAS DataBase Reference) |
| FDA UNII | RMW9V5RW38 |
| NCI Drug Dictionary | trifluridine |
| ATC code | L01BC59,S01AD02 |
| UNSPSC Code | 41116107 |
| NACRES | NA.51 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H341-H351-H361d | |||||||||
| Precautionary statements | P201-P202-P280-P308+P313-P405-P501 | |||||||||
| PPE | dust mask type N95 (US), Eyeshields, Gloves | |||||||||
| Hazard Codes | Xn,Xi | |||||||||
| Risk Statements | 20/21/22-40 | |||||||||
| Safety Statements | 22-36 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | XP2087500 | |||||||||
| Hazard Note | Keep Cold | |||||||||
| HS Code | 29349990 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| Hazard Classifications | Carc. 2 Muta. 2 Repr. 2 |
|||||||||
| Hazardous Substances Data | 70-00-8(Hazardous Substances Data) | |||||||||
| Toxicity | LD50 intraperitoneal in mouse: 1931mg/kg | |||||||||
| NFPA 704 |
|
Trifluridine price More Price(104)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | T2255 | Trifluorothymidine ≥99% (HPLC) | 70-00-8 | 1g | $2080 | 2026-04-30 | Buy |
| Sigma-Aldrich | PHR3150 | Trifluridine pharmaceutical secondary standard, certified reference material | 70-00-8 | 200MG | $341 | 2026-04-30 | Buy |
| Sigma-Aldrich | BP1126 | Trifluridine British Pharmacopoeia (BP) Reference Standard | 70-00-8 | 100MG | $287 | 2026-04-30 | Buy |
| Sigma-Aldrich | 1686309 | Trifluridine United States Pharmacopeia (USP) Reference Standard | 70-00-8 | 100mg | $583 | 2026-04-30 | Buy |
| TCI Chemical | T2511 | Trifluorothymidine >98.0%(HPLC)(T) | 70-00-8 | 100mg | $179 | 2026-04-30 | Buy |
Trifluridine Chemical Properties, Uses, Production
Description
Trifluridine (trifluorothymidine, TFT), a fluorinated pyrimidine nucleoside, is an anti-herpesvirus agent and an antitumor antimetabolite agent. It is an analog of thymidine which inhibits thymidylate synthase possesses antiviral and anticancer activity. After phosphorylation by thymidine kinase, it is incorporated into DNA where it induces DNA-damage and interferes with repair enzymes. Enhances frame shift insertion and deletion in CRISPR genome editing in pluripotent stem cells.
Chemical Properties
White to Off-White Solid
Originator
Trifluorothymidine ,Mann,W. Germany,1975
Uses
Trifluridine is used as anti-herpesvirus antiviral agent in ophthalmie preparations.
Indications
Trifluridine (Viroptic) is a fluorinated pyrimidine nucleoside that has in vitro activity against HSV-1 and HSV- 2, vaccinia, and to a lesser extent, some adenoviruses. Activation of trifluridine requires its conversion to the 5 monophosphate form by cellular enzymes.Trifluridine monophosphate inhibits the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) by thymidylate synthetase. In addition, it competes with deoxythymidine triphosphate (dTTP) for incorporation by both viral and cellular DNA polymerases. Trifluridine-resistant mutants have been found to have alterations in thymidylate synthetase specificity.
Definition
ChEBI: Trifluridine is a pyrimidine 2'-deoxyribonucleoside compound having 5-trifluoromethyluracil as the nucleobase. An antiviral drug used mainly in the treatment of primary keratoconjunctivitis and recurrent epithelial keratitis. It has a role as an antiviral drug, an antimetabolite, an EC 2.1.1.45 (thymidylate synthase) inhibitor and an antineoplastic agent. It is a nucleoside analogue, an organofluorine compound and a pyrimidine 2'-deoxyribonucleoside.
brand name
Viroptic (Monarch).
Therapeutic Function
Antiviral (ophthalmic)
General Description
Trifluridine, 5-trifluoromethyl-29-deoxyuridine (Viroptic),is a fluorinated pyrimidine nucleoside that demonstrates invitro inhibitory activity against HSV-1 and HSV-2, CMV,vaccinia, and some adenoviruses.Trifluridine possesses atrifluoromethyl group instead of an iodine atom at the 5-position of the pyrimidine ring.
synthetase, and the biologically generated triphosphatecompetitively inhibits thymidine triphosphate incorporationinto DNA by DNA polymerase. In addition, trifluridine inits triphosphate form is incorporated into viral and cellularDNA, creating fragile, poorly functioning DNA.Trifluridine is approved in the United States for the treatmentof primary keratoconjunctivitis and recurrent epithelialkeratitis caused by HSV types 1 and 2. Topical trifluridineshows some efficacy in patients with acyclovir-resistantHSV cutaneous infections.
Biochem/physiol Actions
Trifluorothymidine is a thymidine analog and is light sensitive. TFT serves as a thymidine kinase substrate to study enzyme specificity and kinetics. Incorporation of phosphorylated TFT into DNA induces damage, making it useful for DNA repair studies. TFT may also be used in the inhibition of thymidylate synthase and in screening mutant thymidine kinase gene.. It elicits antitumor activity in gastrointestinal (GI) cancers and has therapeutic potential to treat herpetic keratitis.
Mechanism of action
Trifluorothymidine is a fluorinated pyridine nucleoside structurally related to idoxuridine. It has been approved by the U.S. FDA and is a potent, specific inhibitor of replication of HSV-1 in vitro. Its mechanism of action is similar to that of idoxuridine. Like other antiherpes drugs, it is first phos-phorylated by thymidine kinase to mono-, di-, and triphosphate forms, which are then incorporated into viral DNA in place of thymidine to stop the formation of late virus mRNA and subsequent synthesis of the virion proteins.
Pharmacokinetics
Trifluorothymidine is a synthetic halogenated pyrimidine nucleoside, first synthesized as an antitumor agent. It inhibits enzymes of the DNA pathway and is incorporated into both cellular and progeny viral DNA, causing faulty transcription of late messenger RNA and the production of incompetent virion protein. It does not require a viral thymidine kinase for monophosphorylation and is far less selective and more toxic than other analogs. It is active against HSV-1 and HSV-2, vaccinia virus, CMV and possibly adenovirus. Trifluorothymidine, when given IV, shows a plasma half-life of 18 minutes and is excreted in the urine either unchanged or as the inactive metabolite 5-carboxyuracil. When applied as a 1% ophthalmic solution, it rapidly enters the aqueous humor of HSV-infected rabbits’ eyes but is cleared within 60–90 min.
Side effects
The most frequent adverse reactions to trifluridine administration
are transient burning or stinging and
palpebral edema. Other adverse reactions include superficial
punctate keratopathy, epithelial keratopathy,
hypersensitivity, stromal edema, irritation, keratitis
sicca, hyperemia, and increased intraocular pressure.
Trifluridine is mutagenic in vitro and carcinogenic
and teratogenic when administered subcutaneously to
animals. Topical trifluridine was not teratogenic in animal
studies. Because it is applied topically in humans,
the likelihood of systemic effects is low.
Synthesis
Trifluridine, 5-trifluoromethyl-1-(2-deoxyribofuranosyl)pyrimidin-2,
4-(1H.3H)-dione (36.1.22), is synthesized from 5-trifluoromethyluracil. This is synthesized
by the following scheme. It begins with trifluoroacetone, from which the oxynitrile (36.1.16)
is synthesized. Acetylation of this product gives the corresponding trifluoroacetate (36.1.16).
Pyrrolysis of this compound gives trifluoromethylacrylonitrile (36.1.17). Adding to this dry
hydrogen bromide in methanol solution in a process of which methanolysis of the nitrile
group takes place the bromide 36.1.19 is obtained, which upon acidic hydrolysis undergoes
heterocyclization to the dihydropyrimidine 36.1.20. Brominating of the obtained dihydropyrimidine with molecular bromine and subsequent dehydrobromination of the resulting product 36.1.21 on heating in dimethylformamide gives 5-trifluoromethyluracil (36.1.22). This is
reacted with 2-deoxy-D-ribos-1-phosphate using the nucleoside phosphorylase enzyme, or
by treating it with hyxamethyldisylazane and then with trichloromethylsilane to make 2,4-
trimethylsilyloxy-5-trifluoromethyl pyrimidine (36.1.23).
Hexamethyldisilazane, which
itself does not form trimethylsilyl ethers, is used because using a combination of two
reagents leads to optimal yield of trimethylsilyl ethers. Reacting the resulting pyrimidine derivative with 3,5-bis-(4-nitrobenzoate)-2-deoxyribofuranosyl chloride in the presence of
mercury (II) acetate makes the corresponding ditrimethylsilyloxy nucleoside, which when
treated with an aqueous solution of potassium iodide to remove the protecting groups. The
resulting product undergoes preliminary purification by chromatography, and then is treated
with a methanol solution of diisopropylamine to remove the 4-nitrobenzoyl protection from
the furanosyl part, giving the desired trifluridine.
Veterinary Drugs and Treatments
Trifluridine (trifluorothymidine; Viroptic) is a pyrimidine nucleoside analog. It is structurally related to 2-deoxythymidine, the natural precursor of DNA synthesis. Trifluridine is poorly absorbed by the cornea and is virostatic. Viroptic interrupts viral replication by substituting “nonsense” pyrimidine analogues. For this reason, a competent surface immunity is necessary to resolve ocular disease, with or without antiviral therapy. A recent in vitro study in which several strains of feline herpes virus were collected from the United States and were used to infect kidney epithelial cells showed that trifluridine was more effective at lower concentrations compared with several other agents. For this reason, trifluridine was the first choice drug employed in the treatment of feline herpes virus ocular disease for many years. Because of the topical toxicity associated with use of trifluridine in cats, its popularity has diminished greatly. In many milder cases, the irritation associated with topical trifluridine is more intense then the inflammation induced by viral infection. Antiviral agents have also been used in the treatment of superficial punctate keratitis in the horse, thought to be associated with equine herpes virus-2 (EHV-2) infection of the cornea.
References
[1] IRENE V. BIJNSDORP. Differential activation of cell death and autophagy results in an increased cytotoxic potential for trifluorothymidine compared to 5-fluorouracil in colon cancer cells†[J]. International Journal of Cancer, 2009, 126 10: 2457-2468. DOI:10.1002/ijc.24943
[2] OLAF H TEMMINK. Trifluorothymidine resistance is associated with decreased thymidine kinase and equilibrative nucleoside transporter expression or increased secretory phospholipase A2.[J]. Molecular Cancer Therapeutics, 2010: 1047-1057. DOI:10.1158/1535-7163.mct-09-0932
[3] NORIHIKO SUZUKI Masakazu F Tomohiro Emura. Mode of action of trifluorothymidine (TFT) against DNA replication and repair enzymes.[J]. International journal of oncology, 2011, 39 1: 263-270. DOI:10.3892/ijo.2011.1003
[4] CHEN YU. Small molecules enhance CRISPR genome editing in pluripotent stem cells.[J]. Cell stem cell, 2015: 142-147. DOI:10.1016/j.stem.2015.01.003
Trifluridine Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Jilin Shengchuang Biotechnology Co., LTD | 13251734045; 13251734045 | 396921152@qq.com | China | 500 | 58 |
| Shanghai YuLue Chemical Co., Ltd. | 021-60345187 13671753212 | lzz841106@aliyun.com | China | 10244 | 55 |
| Innobio Pharm Co.,Ltd | 15600141006 | sales@innovbiopharm.com | China | 117 | 58 |
| Cangzhou Enke Pharma Tech Co.,ltd. | 0317-5106596 15533709196 | 1154424302@qq.com | China | 2502 | 58 |
| Nanjing Jiusinuo Biomedical Technology Co., Ltd. | 19822625399 | njjsn2023@126.com | China | 282 | 58 |
| Zhengzhou Nucleosides Biotech Co., Ltd. | 15981919713 15981919713 | sales@nucleosides.com | China | 471 | 58 |
| Shanghai Chemilab Biotechnology CO.,Ltd | 18116465878 | kmlb@kaimilaibo.com | China | 2414 | 58 |
| Shanghai Boyle Chemical Co., Ltd. | sales@boylechem.com | China | 2915 | 55 | |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
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View Latest Price from Trifluridine manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-17 | Trifluridine
70-00-8
|
1g | 98%min | 1000g | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-08-20 | Trifluridine USP/EP/BP
70-00-8
|
$1.10 | 1g | 99.9% | 100 Tons Min | Dideu Industries Group Limited | |
 |
2026-05-11 | Trifluridine
70-00-8
|
$30.00-65.00 | 99.39% | 10g | TargetMol Chemicals Inc. |
-
- Trifluridine
70-00-8
- $0.00
- 98%min
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Trifluridine USP/EP/BP
70-00-8
- $1.10
- 99.9%
- Dideu Industries Group Limited
-
- Trifluridine
70-00-8
- $30.00-65.00
- 99.39%
- TargetMol Chemicals Inc.
