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Hoechst 33258

Hoechst 33258 Structure
Hoechst 33258 structure
CAS No.
23491-45-4
Chemical Name:
Hoechst 33258
Synonyms
BBIH;BXI-72;H33258;abs9129;Ht-33258;hoe33058;NSC334072;HOE 33258;nsc-322921;33258hoechst
CBNumber:
CB8329486
Molecular Formula:
C25H25ClN6O
Molecular Weight:
460.97
MDL Number:
MFCD00012679
MOL File:
23491-45-4.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-08-20 20:20:01
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Hoechst 33258 Properties

Melting point 314 °C
Density 1.5099 (rough estimate)
refractive index 1.6300 (estimate)
storage temp. -20°C
solubility H2O: 10 mg/mL
form powder
color Yellow to Very Dark Yellow
Water Solubility SOLUBLE
BRN 4088183
Stability Hygroscopic
InChIKey UDJDXLXTHZQAAC-UHFFFAOYSA-N
SMILES CN1CCN(C2C=CC3NC(C4C=CC5NC(C6C=CC(O)=CC=6)=NC=5C=4)=NC=3C=2)CC1.Cl
CAS DataBase Reference 23491-45-4(CAS DataBase Reference)
FDA UNII MHT095273M
EPA Substance Registry System Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4)
UNSPSC Code 41106305
NACRES NA.77

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P305+P351+P338
PPE dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes  Xn
Risk Statements  22-36/38-36/37/38-20/21/22
Safety Statements  26-36-24/25-23
WGK Germany  3
RTECS  SM1140500
F  3-8-10
TSCA  TSCA listed
HS Code  32129000
Storage Class 11 - Combustible Solids
Toxicity dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206,
NFPA 704
0
2 0

Hoechst 33258 price More Price(86)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Membrane-permeable, adenine-thymine-specific fluorescent stain. 23491-45-4 1g $1060 2026-04-30 Buy
Sigma-Aldrich 14530 bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 23491-45-4 100mg $117 2026-04-30 Buy
Sigma-Aldrich 14530 bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 23491-45-4 500mg $414 2026-04-30 Buy
TCI Chemical H1343 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate[for Biochemical Research] >98.0%(HPLC) 23491-45-4 25mg $55 2026-04-30 Buy
TCI Chemical B6236 Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining] 23491-45-4 1SET $30 2026-04-30 Buy
Product number Packaging Price Buy
382061 1g $1060 Buy
14530 100mg $117 Buy
14530 500mg $414 Buy
H1343 25mg $55 Buy
B6236 1SET $30 Buy

Hoechst 33258 Chemical Properties,Uses,Production

Chemical Properties

yellow to yellow-green powder

Uses

Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence

Uses

Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.

Uses

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =346 nm
Emission max. =460 nm

General Description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

storage

+4°C (desiccate)

Hoechst 33258 Preparation Products And Raw materials

Raw materials

Preparation Products

Hoechst 33258 Suppliers

Global( 379)Suppliers
Supplier Tel Email Country ProdList Advantage
Nanjing UltraPure Biotech Co., Ltd. 025-82263158 13851993283 2842979086@qq.com China 243 58
G-CLONE 400-910-1997 15330005590 3546414794@qq.com China 9957 58
Hangzhou Kaitan Technology Co., Ltd. 18668996177 249358374@qq.com China 74 58
J & K SCIENTIFIC LTD. 18210857532 18210857532 jkinfo@jkchemical.com China 96815 76
Meryer (Shanghai) Chemical Technology Co., Ltd. 4006356688 18621169109 market03@meryer.com China 40202 62
TCI (Shanghai) Development Co., Ltd. 021-67121386 Sales-CN@TCIchemicals.com China 24512 81
Beijing Ouhe Technology Co., Ltd 13552068683 13522913783 2355560935@qq.com China 11777 60
Adamas Reagent, Ltd. 400-6009262 15618770481 yhx@titansci.com China 14098 59
Shanghai Hanhong Scientific Co.,Ltd. 021-54306202 13764082696 info@hanhongsci.com China 42917 64
Shandong Xiya Chemical Co., Ltd 13355009207 13355009207 3007715519@qq.com China 18722 57
Supplier Advantage
Nanjing UltraPure Biotech Co., Ltd. 58
G-CLONE 58
Hangzhou Kaitan Technology Co., Ltd. 58
J & K SCIENTIFIC LTD. 76
Meryer (Shanghai) Chemical Technology Co., Ltd. 62
TCI (Shanghai) Development Co., Ltd. 81
Beijing Ouhe Technology Co., Ltd 60
Adamas Reagent, Ltd. 59
Shanghai Hanhong Scientific Co.,Ltd. 64
Shandong Xiya Chemical Co., Ltd 57

View Lastest Price from Hoechst 33258 manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Hoechst 33258 trihydrochloride pictures 2026-08-20 Hoechst 33258 trihydrochloride
23491-45-4
 $53.00 99.47% 10g TargetMol Chemicals Inc.
Hoechst 33258 pictures 2019-07-14 Hoechst 33258
23491-45-4
 $1.00 1KG 99% 25kg Career Henan Chemical Co

Hoechst 33258 Spectrum

Hoechst 33258(23491-45-4)1HNMRHoechst 33258(23491-45-4)FT-IR

23491-45-4(Hoechst 33258)Related Search:

Apoptosis-HOECHST staining kit Thiophanate-methyl Mitochondrial Membrane Potential Assay Kit with JC-1 Bensulfuron methyl Kresoxim-methyl
Cy5.5 Methyl acetate Methylparaben Methyl acrylate Metsulfuron methyl
Hoechst 33342 Methyl Methyl bromide HOECHST 33258 Parathion-methyl
2'-(4-Methacryloxyphenyl)-5-(4-methyl-1-piper-azinyl)-2,5'-bibenzimidazolyl trihydrochloride Bisbenzimide h33258 Hoechst 33258

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Bis-BenzimideFluorochrome p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride Hoechst(R) 33258,98% bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride 4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride Pibenzimol HCl Ht-33258 Bisbenzimide H33258 p-(5-(5-(4-Methyl-1-pip bisBenzimide H 33258,2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydroc Bis Benzimide Hoechst H 33258 BBIH 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride Hydrate [for BiocheMical Research] Hearst fluorescence fuels, 33258 33258hoechst 4-(5-(4-methyl-1-piperazinyl)(2,5’-bi-1h-benzimidazol)-2’-yl)-phenotrihy 4-[5-(4-methyl-1-piperazinyl)[2,5’-bi-1h-benzimidazol]-2’-yl]-phenotrihydr bisbenzimidazoletrihydrochloride bisbenzimidehydrochloride BISBENZIMIDE H 33258 FLUOROCHROME BISBENZIMIDE H 33258 FLUOROCHROME PACKAG BISBENZIMIDE H 33258, FOR FLUORESCENCE HOECHST 33258,98% BXI-72 NSC334072 Hoechst 33258 solution Bisbenzimide H33258 Fluorochrome Trihydrochloride 〔HOE 33258〕 Bisbenzimide H33258 Fluorochrome Trihydrochloride DMSO Solution (1mg/ml) Bisbenzimide (Hoechst 33258) Hoechst 33258 (trihydrochloride) Hoechst 33258 *20 mM solution in water* Hoechst 33258 3HCl Hoechst 33258 solution, 1mg/mL in H2O Hoechst 33258 *CAS 23491-45-4* Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride hoe33058 hoechstdye33258 nsc-322921 HOE 33258 HOECHST NO 33258 HOECHST STAIN SOLUTION HOECHST 33258 H33258 BISBENZIMIDE TRIHYDROCHLORIDE BISBENZIMIDE H 33258 BISBENZIMIDE H 33258 FLUOROCHROME, TRIHYDROCHLORIDE BISBENZIMIDE H 33258 TRIHYDROCHLORIDE BISBENZIMIDE 2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI(1H-BENZIMIDAZOLE) TRIHYDROCHLORIDE 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOLYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE TRIHYDROCHLORIDE 2-[-2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE, 3HCL 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)BENZIMIDAZOLE TRIHYDROCHLORIDE p-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride BISBENZIMIDE HOECHST NO 33258TRIHYDROCHL ORIDE