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Hoechst 33258

Hoechst 33258 Structure
Hoechst 33258 structure
CAS No.
23491-45-4
Chemical Name:
Hoechst 33258
Synonyms
BBIH;BXI-72;H33258;abs9129;Ht-33258;hoe33058;NSC334072;HOE 33258;nsc-322921;33258hoechst
CBNumber:
CB8329486
Molecular Formula:
C25H25ClN6O
Molecular Weight:
460.97
MDL Number:
MFCD00012679
MOL File:
23491-45-4.mol
MSDS File:
SDS
Last updated:2026-02-02 18:10:39
Product description Number Pack Size Price
Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Membrane-permeable, adenine-thymine-specific fluorescent stain. 382061 1g $992
bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 14530 100mg $109
bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 14530 500mg $385
2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate[for Biochemical Research] >98.0%(HPLC) H1343 25mg $65
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining] B6236 1SET $37
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Hoechst 33258 Properties

Melting point 314 °C
Density 1.5099 (rough estimate)
refractive index 1.6300 (estimate)
storage temp. -20°C
solubility H2O: 10 mg/mL
form powder
color Yellow to Very Dark Yellow
Water Solubility SOLUBLE
BRN 4088183
Stability Hygroscopic
InChIKey UDJDXLXTHZQAAC-UHFFFAOYSA-N
SMILES CN1CCN(C2C=CC3NC(C4C=CC5NC(C6C=CC(O)=CC=6)=NC=5C=4)=NC=3C=2)CC1.Cl
CAS DataBase Reference 23491-45-4(CAS DataBase Reference)
FDA UNII MHT095273M
EPA Substance Registry System Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4)
UNSPSC Code 41106305
NACRES NA.77

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P305+P351+P338
PPE dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes  Xn
Risk Statements  22-36/38-36/37/38-20/21/22
Safety Statements  26-36-24/25-23
WGK Germany  3
RTECS  SM1140500
F  3-8-10
TSCA  TSCA listed
HS Code  32129000
Storage Class 11 - Combustible Solids
Toxicity dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206,
NFPA 704
0
2 0

Hoechst 33258 price More Price(42)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Membrane-permeable, adenine-thymine-specific fluorescent stain. 23491-45-4 1g $992 2025-07-31 Buy
Sigma-Aldrich 14530 bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 23491-45-4 100mg $109 2025-07-31 Buy
Sigma-Aldrich 14530 bisBenzimide H 33258 for fluorescence, ≥98.0% (HPLC) 23491-45-4 500mg $385 2025-07-31 Buy
TCI Chemical H1343 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate[for Biochemical Research] >98.0%(HPLC) 23491-45-4 25mg $65 2025-07-31 Buy
TCI Chemical B6236 Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining] 23491-45-4 1SET $37 2025-07-31 Buy
Product number Packaging Price Buy
382061 1g $992 Buy
14530 100mg $109 Buy
14530 500mg $385 Buy
H1343 25mg $65 Buy
B6236 1SET $37 Buy

Hoechst 33258 Chemical Properties,Uses,Production

Chemical Properties

yellow to yellow-green powder

Uses

Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence

Uses

Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.

Uses

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =?346 nm
Emission max. =?460 nm

General Description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

storage

+4°C (desiccate)

Hoechst 33258 Preparation Products And Raw materials

Raw materials

Preparation Products

Hoechst 33258 Suppliers

Global( 375)Suppliers
Supplier Tel Email Country ProdList Advantage
ATK CHEMICAL COMPANY LIMITED 		+undefined-21-51877795 ivan@atkchemical.com China 33024 60
career henan chemical co 		+86-0371-86658258 +8613203830695 sales@coreychem.com China 29833 58
CONIER CHEM AND PHARMA LIMITED 		+8618523575427 sales@conier.com China 49977 58
TargetMol Chemicals Inc. 		+1-781-999-5354; +17819995354 marketing@targetmol.com United States 32470 58
Hefei TNJ Chemical Industry Co.,Ltd. 		+86-0551-65418671 +8618949823763 sales@tnjchem.com China 34563 58
ANHUI WITOP BIOTECH CO., LTD 		+8615255079626 eric@witopchemical.com China 23541 58
Hefei Hirisun Pharmatech Co., Ltd 		+8615056975894 shawn@hirisunpharm.com CHINA 9911 58
changzhou huayang technology co., ltd 		+8615250961469 2571773637@qq.com China 9651 58
InvivoChem 		+1-708-310-1919 +1-13798911105 sales@invivochem.cn United States 6391 58
HANGZHOU LEAP CHEM CO., LTD. 		+86-571-87711850 market18@leapchem.com China 24597 58
Supplier Advantage
ATK CHEMICAL COMPANY LIMITED 		60
career henan chemical co 		58
CONIER CHEM AND PHARMA LIMITED 		58
TargetMol Chemicals Inc. 		58
Hefei TNJ Chemical Industry Co.,Ltd. 		58
ANHUI WITOP BIOTECH CO., LTD 		58
Hefei Hirisun Pharmatech Co., Ltd 		58
changzhou huayang technology co., ltd 		58
InvivoChem 		58
HANGZHOU LEAP CHEM CO., LTD. 		58

View Lastest Price from Hoechst 33258 manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Hoechst 33258 trihydrochloride pictures 2026-02-02 Hoechst 33258 trihydrochloride
23491-45-4
 US $53.00 / mg 99.47% 10g TargetMol Chemicals Inc.
Hoechst 33258 pictures 2019-07-14 Hoechst 33258
23491-45-4
 US $1.00 / KG 1KG 99% 25kg Career Henan Chemical Co

Hoechst 33258 Spectrum

Hoechst 33258(23491-45-4)1HNMRHoechst 33258(23491-45-4)FT-IR

23491-45-4(Hoechst 33258)Related Search:

Bensulfuron methyl Kresoxim-methyl METSULFURON METHYL Cy5.5 Methyl acetate
Methylparaben Methyl acrylate Apoptosis-HOECHST staining kit Thiophanate-methyl Mitochondrial Membrane Potential Assay Kit with JC-1
Hoechst 33342 Methyl Parathion-methyl Methyl bromide hoechst 33258 (bisbenzimide)
HOECHST 33258 TRIHYDROCHLORIDE PENTAHYDRATE,HOECHST 33258, PENTAHYDRATE O-METHACRYLOYL HOECHST 33258 Hoechst 33258

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Bis-BenzimideFluorochrome p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride Hoechst(R) 33258,98% bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride 4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride Pibenzimol HCl Ht-33258 Bisbenzimide H33258 p-(5-(5-(4-Methyl-1-pip bisBenzimide H 33258,2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydroc Bis Benzimide Hoechst H 33258 BBIH 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride Hydrate [for BiocheMical Research] Hearst fluorescence fuels, 33258 33258hoechst 4-(5-(4-methyl-1-piperazinyl)(2,5’-bi-1h-benzimidazol)-2’-yl)-phenotrihy 4-[5-(4-methyl-1-piperazinyl)[2,5’-bi-1h-benzimidazol]-2’-yl]-phenotrihydr bisbenzimidazoletrihydrochloride bisbenzimidehydrochloride BISBENZIMIDE H 33258 FLUOROCHROME BISBENZIMIDE H 33258 FLUOROCHROME PACKAG BISBENZIMIDE H 33258, FOR FLUORESCENCE HOECHST 33258,98% BXI-72 NSC334072 Hoechst 33258 solution Bisbenzimide H33258 Fluorochrome Trihydrochloride 〔HOE 33258〕 Bisbenzimide H33258 Fluorochrome Trihydrochloride DMSO Solution (1mg/ml) Bisbenzimide (Hoechst 33258) Hoechst 33258 (trihydrochloride) Hoechst 33258 *20 mM solution in water* Hoechst 33258 3HCl Hoechst 33258 solution, 1mg/mL in H2O Hoechst 33258 *CAS 23491-45-4* Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride hoe33058 hoechstdye33258 nsc-322921 HOE 33258 HOECHST NO 33258 HOECHST STAIN SOLUTION HOECHST 33258 H33258 BISBENZIMIDE TRIHYDROCHLORIDE BISBENZIMIDE H 33258 BISBENZIMIDE H 33258 FLUOROCHROME, TRIHYDROCHLORIDE BISBENZIMIDE H 33258 TRIHYDROCHLORIDE BISBENZIMIDE 2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI(1H-BENZIMIDAZOLE) TRIHYDROCHLORIDE 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOLYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE TRIHYDROCHLORIDE 2-[-2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE, 3HCL 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)BENZIMIDAZOLE TRIHYDROCHLORIDE p-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride BISBENZIMIDE HOECHST NO 33258TRIHYDROCHL ORIDE