2-(4-アミジノフェニル)-1H-インドール-6-カルボアミジン·2塩酸塩 化学特性,用途語,生産方法
外観
黄色〜黄緑色, 粉末
溶解性
水及びメタノールに溶けやすく、エタノールにやや溶けにくく、アセトンに極めて溶けにくい。
用途
電気泳動におけるRNA存在下での2本鎖DNAの検出。
説明
DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-
T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.
化学的特性
Yellow Solid
使用
4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
一般的な説明
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
参考文献
[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:
10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:
10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:
10.1038/srep31417.
2-(4-アミジノフェニル)-1H-インドール-6-カルボアミジン·2塩酸塩 上流と下流の製品情報
原材料
準備製品