p-Nitrophenyl phosphate di(Tris) salt

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p-Nitrophenyl phosphate di(Tris) salt Basic information
Product Name:p-Nitrophenyl phosphate di(Tris) salt
Synonyms:p-nitrophenyl dihydrogen phosphate, compound with 2-amino-2-(hydroxymethyl)propane-1,3-diol (1:2);p-Nitrophenylphosphate bis[tris-(hydroxymethylamino)-methane] salt;P-NITROPHENYL PHOSPHATE DITRIS;4-NitrophenylphosphateBis(Tris)Salt;PNPP-TRIS;PNPP, 2TRIS;P-NPP, DITRIS SALT;l)-1,3-propanediol(1:2)
CAS:68189-42-4
MF:C14H28N3O12P
MW:461.36
EINECS:269-198-6
Product Categories:substrate;Alkaline Phosphatase;Alkaline PhosphataseEnzyme Substrates;Alphabetical ListingELISA Substrates;Detection Substrates;Enzyme Substrates;Powders;Substrates by Enzyme
Mol File:68189-42-4.mol
p-Nitrophenyl phosphate di(Tris) salt Structure
p-Nitrophenyl phosphate di(Tris) salt Chemical Properties
storage temp. 2-8°C
solubility H2O: 0.1 g/mL, clear
form Off-white to pale yellow solid
color White to Orange to Green
Water Solubility water: soluble
BRN 1578142
InChI1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChIKeyXXAXKCWOTRABOW-UHFFFAOYSA-N
SMILESNC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
LogP-2.16 at 22℃
CAS DataBase Reference68189-42-4(CAS DataBase Reference)
EPA Substance Registry SystemMono(p-nitrophenyl) phosphate 2-amino-2-(hydroxymethyl)-1,3-propanediol salt (1:2) (68189-42-4)
Safety Information
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36-24/25
WGK Germany 3
F 8-10-21
TSCA TSCA listed
HS Code 29199000
Storage Class11 - Combustible Solids
MSDS Information
ProviderLanguage
SigmaAldrich English
p-Nitrophenyl phosphate di(Tris) salt Usage And Synthesis
Uses4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biological Activityp-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.
p-Nitrophenyl phosphate di(Tris) salt Preparation Products And Raw materials
Tag:p-Nitrophenyl phosphate di(Tris) salt(68189-42-4) Related Product Information
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