|
|
| | 3-Methyl-2-([1-[3-(trimethylammonio)propyl]-4(1H)-quinolinylidene]methyl)-1,3-benzothiazol-3-ium diiodide Basic information |
| | 3-Methyl-2-([1-[3-(trimethylammonio)propyl]-4(1H)-quinolinylidene]methyl)-1,3-benzothiazol-3-ium diiodide Chemical Properties |
| Melting point | >250℃ | | solubility | Soluble in dimethyl sulfoxide | | form | powder | | color | Red-brown | | Appearance | Green solution | | ex/em | 515/531 nm | | ε(extinction coefficient) | 62800 cm−1M−1 | | Φ(quantum yield) | 0.25 | | λmax | 515 nm | | Biological Applications | Nucleic acid hybridization; nucleic acid assay; nucleic acid fragment sizing; nucleic acid sequencing; detecting nucleic acids,cells,Human papilloma virus (HPV),microstructures; counting embryoblasts,leukocytes; monitoring |
| | 3-Methyl-2-([1-[3-(trimethylammonio)propyl]-4(1H)-quinolinylidene]methyl)-1,3-benzothiazol-3-ium diiodide Usage And Synthesis |
| Description | TO-TAP-1 (Thiazole Orange Monomer, also known as TO-PRO®-1) is a green fluorescent carbocyanine monomeric dye. TO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make TO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TO-TAP-1 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture. | | Chemical Properties | Appearance: Green solution
ex/em: 515/531 nm
Extinction coefficient (ε): 62800 cm1M1
Quantum yield (Φ): 0.25 | | Uses | TO-PRO 1 is a DNA binding fluorochrome, that atached to the surface of the Feraheme (FH) nanoparticle (NP), to obtain a fluorochrome-functionalized NP. TO-PRO 1 binds DNA through intercalation, and acts as a vital fluorochrome for necrotic cells[1]. | | Definition | ChEBI: A C1 cyanine dye having benzothiazolium-2-yl and quinolinium-4-yl substituents. | | References | [1] Hoonsung Cho, et al. Fluorochrome-functionalized nanoparticles for imaging DNA in biological systems. ACS Nano. 2013 Mar 26;7(3):2032-41. DOI:10.1021/nn305962n | | Abs/Em Maxima | 513/533 nm |
| | 3-Methyl-2-([1-[3-(trimethylammonio)propyl]-4(1H)-quinolinylidene]methyl)-1,3-benzothiazol-3-ium diiodide Preparation Products And Raw materials |
|