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| | MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Basic information |
| Product Name: | MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 | | Synonyms: | MOCAC-PRO-LEU-GLY-LEU-N3-[2,4-DINITROPHENYL]-L-2,3-DIAMINOPROPIONYL-ALA-ARG-NH2;MMP-2/MMP-7 SUBSTRATE;MMP-2/MMP-7 SUBSTRATE, FLUOROGENIC;MCA-PRO-LEU-GLY-LEU-DPA-ALA-ARG-NH2;MCA-PLGL-DAP(DNP)-ALA-ARG-NH2;MCA-PLGL-DAP(DNP)-AR-NH2;FLUORESCENT PEPTIDE SUBSTRATE I;7-methoxycoumarin-4-acetyl-pro-leu-*gly-leu-B-(2, | | CAS: | 140430-53-1 | | MF: | C49H68N14O15 | | MW: | 1093.15 | | EINECS: | | | Product Categories: | peptides;peptide | | Mol File: | 140430-53-1.mol |  |
| | MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Chemical Properties |
| density | 1.48±0.1 g/cm3(Predicted) | | storage temp. | −20°C | | solubility | 20% acetic acid: 1mg/mL | | form | Solid | | pka | 12.99±0.46(Predicted) | | color | Light yellow to yellow | | Appearance | Yellow Powder | | InChIKey | RQASLOJQHHELIQ-QJSKOQLASA-N |
| WGK Germany | 3 | | Storage Class | 11 - Combustible Solids |
| | MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Usage And Synthesis |
| Description | 7-Methoxycoumarin-4-acetyl-P-L-G-L-β-(2,4-dinitrophenylamino)A-R amide is a fluorogenic substrate for matrix metalloproteinases (MMPs). It is ceaved by an MMP to release the fluorescent moiety 7-methoxycoumarin-4-acetyl (Mca), which can be used to quantify MMP activity. | | Uses | MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues[1][2]. | | Biological Activity | Cell permeable: no', 'Primary Target
Fluorogenic substrate for matrix metalloproteinases (MMPs)', 'Product does not compete with ATP.', 'Reversible: no', 'kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M-¹ sec-¹ for MMP-2 | | References | [1] Oneda H, et al. Substrate-dependent activation of thermolysin by salt. Biosci Biotechnol Biochem. 2004 Aug;68(8):1811-3. DOI:10.1271/bbb.68.1811
[2] Oneda H, et al. Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin). J Biochem. 2003 May;133(5):571-6. DOI:10.1093/jb/mvg073 | | Sequene | Mca-PLGL-Dap(Dnp)-AR-NH2 |
| | MCA-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Preparation Products And Raw materials |
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