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| | PAMP-12 (human, mouse, rat, porcine, bovine) (trifluoroacetate salt) Basic information |
| | PAMP-12 (human, mouse, rat, porcine, bovine) (trifluoroacetate salt) Chemical Properties |
| solubility | DMF: 50 mg/ml
DMSO: 10 mg/mlPBS (pH 7.2): 10 mg/ml |
| | PAMP-12 (human, mouse, rat, porcine, bovine) (trifluoroacetate salt) Usage And Synthesis |
| Description | Proadrenomedullin N-terminal 12 peptide (PAMP-12) is an endogenous peptide fragment found in the adrenal medulla that corresponds to amino acids 9-20 of human PAMP-20 and is involved in hypotension.1,2,3 It is an agonist of MAS-related G protein-coupled receptor family member X2 (MRGPRX2).2 PAMP-12 inhibits forskolin-induced cAMP accumulation in CHO cells expressing human MRGPRX2 (EC50 = 57.2 nM). It selectively induces calcium mobilization in CHO cells expressing MRGPRX2 (EC50 = 41 nM) over those expressing MRGPRX1, MRGPRX3, or MRGPRX4 at 1 µM. PAMP-12 is an antagonist of nicotinic acetylcholine receptors (nAChRs) that inhibits carbachol-induced catecholamine release (IC50 = 1.3 µM) and calcium and sodium influx (IC50s = 0.39 and 0.87 µM, respectively), but not histamine-induced catecholamine release or calcium and sodium influx (IC50s = >1 µM for all), in primary bovine adrenal chromaffin cells.3 It reduces mean arterial blood pressure in normotensive rats when administered at doses ranging from 10 to 50 nmol/kg.1WARNING This product is not for human or veterinary use. | | References | [1] KENJI KUWASAKO . Purification and characterization of PAMP-12 (PAMP[9–20]) in porcine adrenal medulla as a major endogenous biologically active peptide[J]. FEBS Letters, 1997, 414 1: Pages 105-110. DOI: 10.1016/s0014-5793(97)00971-x
[2] MASAZUMI KAMOHARA . Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides[J]. Biochemical and biophysical research communications, 2005, 330 4: Pages 1146-1152. DOI: 10.1016/j.bbrc.2005.03.088
[3] HIDEYUKI KOBAYASHI . Selective inhibition of nicotinic cholinergic receptors by proadrenomedullin N-terminal 12 peptide in bovine adrenal chromaffin cells[J]. Molecular Brain Research, 2001, 87 2: Pages 175-183. DOI: 10.1016/s0169-328x(01)00011-0 |
| | PAMP-12 (human, mouse, rat, porcine, bovine) (trifluoroacetate salt) Preparation Products And Raw materials |
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