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| | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Basic information |
| Product Name: | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT | | Synonyms: | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT;N-TOSYLGLYCYL-L-PROLYL-L-ARGININE 4-NITROANILIDE ACETATE;N-tosylglycyl-L-prolyl-L-arginine 4-nitroanilide acetat;N-tosylglycyl-L-prolyl-N-(p-nitrophenyl)-L-argininamide monoacetate;N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE*ACE TATE;N-Tosyl-Gly-Pro-Arg 4-nitroanilide acetate;n-tosylglycyl-l-prolyl-l-arginine 4-nitroanilide acetate salt;Tosyl-Gly-Pro-Arg-(4-nitrophenyl)NH2·acetic acid | | CAS: | 86890-95-1 | | MF: | C28H38N8O9S | | MW: | 662.71 | | EINECS: | 289-285-2 | | Product Categories: | | | Mol File: | 86890-95-1.mol |  |
| | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Chemical Properties |
| storage temp. | −20°C | | solubility | DMF: 20 mg/ml; DMSO: 3 mg/ml; PBS (pH 7.2): 3 mg/ml | | form | A solid |
| | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Usage And Synthesis |
| Description | N-(p-Tosyl)-GPR-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity. | | Uses | N-(p-Tosyl)-GPR-pNA acetate (Chromozym-TH) is a chromogenic substrate targeting the synthetic peptides Hirunorm IV and Hirunorm V and can be used to detect the dissociation constants (KI) of both peptides. Hirunorm IV and Hirunorm V are reversible inhibitors of amidolytic thrombin activity. By varying the peptide concentration at a fixed concentration of the chromogenic substrate N-(p-Tosyl)-GPR-pNA acetate, the dissociation constants determined were 0.134 nM (Hirunorm IV) and 0.245 nM (Hirunorm V)[1]. | | References | [1] Cappiello M, et al. Kinetics of human thrombin inhibition by two novel peptide inhibitors (Hirunorm IV and Hirunorm V). Biochem Pharmacol. 1996 Oct 25;52(8):1141-6. DOI:10.1016/0006-2952(96)00388-7 |
| | N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT Preparation Products And Raw materials |
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