|
|
| | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE Basic information |
| Product Name: | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE | | Synonyms: | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE;3ALPHA-HYDROXYSTEROID: NAD[P]+ OXIDOREDUCTASE;3alpha-hydroxysteroid dehydrogenase F. pseu. test. ~9 U/mg;3alpha-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni;Dehydrogenase, 3alpha-hydroxy steroid;HYDROXYSTEROID OXIDOREDUCTASE;3ALPHA-HYDROXYSTEROID DEHYDROGENASE F. &;3A-HYDROXYSTEROID DEHYDROGENASE FROM*PSE UDOMONAS TE | | CAS: | 9028-56-2 | | MF: | | | MW: | 0 | | EINECS: | 232-839-5 | | Product Categories: | Oxidoreductases;Specialty Enzymes | | Mol File: | Mol File | ![3ALPHA-HYDROXYSTEROID DEHYDROGENASE Structure]() |
| | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE Chemical Properties |
| storage temp. | -20°C | | form | ammonium sulfate suspension | | color | White to off-white | | biological source | bacterial (Pseudomonas testosteroni) | | Specific Activity | ≥15units/mg protein | | Cosmetics Ingredients Functions | SKIN CONDITIONING |
| Safety Statements | 24/25 | | WGK Germany | 3 | | HS Code | 35079090 | | Storage Class | 11 - Combustible Solids |
| | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE Usage And Synthesis |
| Description | 3α-Hydroxysteroid dehydrogenase is a key enzyme catalyzing steroid metabolism. Its core function is to reversibly catalyze the oxidation of 3α-hydroxysteroids to 3-oxosteroids, using NAD+ or NADP+ as cofactors. As a protein, its molecular weight and optimal reaction conditions vary depending on the source (e.g., human, rat, or bacterial) and subtype. For example, some human subtypes prefer to use NADPH as a cofactor and exhibit optimal activity in a near-neutral environment with a pH of 6.8–7.4. The purified product is typically a white powder. | | Chemical Properties | Powder | | Uses | 3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
- in the preparation of composite electrode platform to for the determination of androsterone
- in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
- for total bile salts assay using an enzymatic method
| | Application | 3α-Hydroxysteroid dehydrogenase (3α-HSD) plays a crucial physiological role in vivo, responsible for inactivating circulating steroid hormones and regulating their activity in target tissues (especially the liver), thus participating in steroid hormone clearance. In vitro, purified 3α-HSD is widely used as a biochemical reagent, primarily for clinical diagnosis and research. For example, it can be used to determine enzyme activity levels for quantitative analysis of bile acid or certain steroid hormone levels. Furthermore, it is an important tool for studying the mechanisms of action of nonsteroidal anti-inflammatory drugs (NSAIDs) and progestins, as these drugs are often found to be inhibitors of this enzyme. | | Biochem/physiol Actions | 3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action. |
| | 3ALPHA-HYDROXYSTEROID DEHYDROGENASE Preparation Products And Raw materials |
|