| Company Name: |
Shanghai Universal Biotech Co.,Ltd
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| Tel: |
15921930842 15921930842 |
| Email: |
yh-wang@univ-bio.com |
| Products Intro: |
Product Name:Active Rho Detection Kit
Package:1Kit Remarks: 见优宁维官网
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| Company Name: |
Cell Signaling Technology Inc
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| Tel: |
21-80243558 86218024 |
| Email: |
info@cellsignal.cn |
| Products Intro: |
Product Name:Active Rho Detection Kit
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| | Active Rho Detection Kit Chemical Properties |
| | Active Rho Detection Kit Usage And Synthesis |
| Description | The Active Rho Detection Kit provides all reagents necessary for measuring activation of Rho GTPase in the cell. GST-Rhotekin-RBD fusion protein is used to bind the activated form of GTP-bound Rho, which can then be immunoprecipitated with glutathione resin. Rho activation levels are then determined by western blot using a Rho Rabbit Antibody. | | Background | The Ras superfamily of small GTP-binding proteins comprise a large class of proteins that can be classified into at least five families based on their sequence and functional similarities: Ras, Rho, Rab, Arf, and Ran. These small G proteins have both GDP/GTP-binding and GTPase activities and function as binary switches in diverse cellular and developmental events that include cell cycle progression, cell survival, actin cytoskeletal organization, cell polarity and movement, and vesicular and nuclear transport. An upstream signal stimulates the dissociation of GDP from the GDP-bound form, which leads to the binding of GTP and formation of the GTP-bound form. The activated G protein then goes through a conformational change in its downstream effector-binding region, leading to the binding and regulation of downstream effectors. This activation can be switched off by the intrinsic GTPase activity, which hydrolyzes GTP to GDP and releases the downstream effectors. These intrinsic guanine nucleotide exchange and GTP hydrolysis activities of Ras superfamily proteins are also regulated by guanine nucleotide exchange factors that promote formation of the active GTP-bound form and GTPase activating proteins that return the GTPase to its GDP-bound inactive form.Rho family small GTPases, including Rho, Rac, and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors, which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins, which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor. RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules. | | References | [1] Takai, Y. et al. (2001) Physiol Rev 81, 153-208.
[2] Colicelli, J. (2004) Sci STKE 2004, RE13.
[3] Wennerberg, K. et al. (2005) J Cell Sci 118, 843-6.
[4] Vigil, D. et al. (2010) Nat Rev Cancer 10, 842-57.
[5] DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
[6] Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
[7] Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9. |
| | Active Rho Detection Kit Preparation Products And Raw materials |
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