| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Protein L
Purity:>95% (SDS-PAGE) Package:5mg Remarks:P3101
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| Company Name: |
Biolab Reagents
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| Tel: |
027-65279366 18108604356 |
| Email: |
products@biolabreagent.com |
| Products Intro: |
Product Name:Protein L
Purity:95% SDS-PAGE Package:1mg;100ug
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| Company Name: |
ACROBiosystems
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| Tel: |
13121935750 |
| Email: |
jiaxin.zhao@acrobiosystems.com |
| Products Intro: |
Product Name:Protein L
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| Company Name: |
Enzo Biochem Inc
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| Tel: |
Enzo Biochem Inc. 13797054060 |
| Email: |
Enzoname@qq.com |
| Products Intro: |
Product Name:Protein-L
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| Product Name: | PROTEIN L | | Synonyms: | PROTEIN L;Protein L from Peptostreptococcus magnus | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![PROTEIN L Structure]() |
| | PROTEIN L Chemical Properties |
| storage temp. | 2-8°C | | form | essentially salt-free, lyophilized powder |
| | PROTEIN L Usage And Synthesis |
| Uses | Protein L from Peptostreptococcus magnus is suitable for use in indirect ELISA (1:100,000). This product may also be used for immunohistochemistry in rat kidney sections . | | General Description | Protein L is an immunoglobulin light chain-binding protein. It is expressed by a few anaerobic bacterial species, such as Peptostreptococcus magnus. | | Biochem/physiol Actions | Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding sites of Ig′s. This means that Protein L binds to a wider range of Ig classes and subclasses from a variety of species than any other commercially available Ig binding protein. It contains four Ig binding domains (B-domains). It may be used for the purification of IgG, IgM, IgA, IgE and IgD containing κ light chains and for the purification of Fab and scFv fragments containing κ light chains. It may be conjugated to a solid support for affinity purification or conjugated to marker molecules for use in detection. Protein L does not bind to bovine, sheep or goat immunoglobulins. For this reason it may be used for the purification of monoclonal antibodies from media supplemented with bovine serum albumin or fetal calf serum. It can also be used to purify human or mouse antibodies from goat, bovine or sheep proteins. A comprehensive list of binding properties is found in the product data sheet. | | Description | Protein-L Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 365 amino acids and having a molecular mass of 40.5 kDa but it migrates with an apparent molecular mass of 45 kDa in SDS-PAGE.The Protein-L is purified by proprietary chromatographic techniques. | | Source | Escherichia Coli | | Background | The recombinant Protein L is genetically engineered protein and holds 5 IgG-binding regions of protein L and it has the exclusive capability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding. The recombinant Protein L is perfect for purification of polyclonal or monoclonal IgG antibodies and it binds to human, mouse, rat and pig IgG. |
| | PROTEIN L Preparation Products And Raw materials |
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