| Company Name: |
Quality Control Solutions Ltd.
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| Tel: |
0755-66853366 13670046396 |
| Email: |
orders@qcsrm.com |
| Products Intro: |
Product Name:5-CFDA-AM
CAS:124412-00-6
Purity:95% HPLC Package:10mg;25mg;50mg;100mg
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| Company Name: |
Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.
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| Tel: |
0755-23251735 13302967066 |
| Email: |
sales@geneseqtools.com |
| Products Intro: |
Product Name:5-Carboxyfluorescein diacetate acetoxymethyl ester;5-CFDA, AM
CAS:124412-00-6
Purity:98% HPLC Package:1mg;5mg;100mg;定制包装
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| | 5-CFDA, AM Basic information |
| Product Name: | 5-CFDA, AM | | Synonyms: | 5-CARBOXYFLUORESCEIN DIACETATE, ACETOXYMETHYL ESTER;5-CFDA, AM;5-(AND-6)-CARBOXY SNARF(R)-1, ACETOXYMETHYL ESTER, ACETATE;5-Carboxyfluorescein diacetate acetoxylmethyl ester;3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid (acetyloxy)methyl ester;5-CFDA-AM [5-carboxyfluorescein diacetate, acetoxymethyl ester];YDBUJZYYYBVSEF-UHFFFAOYSA-N;5-CFDA-AM, 90%, single isomer | | CAS: | 124412-00-6 | | MF: | C28H20O11 | | MW: | 532.45 | | EINECS: | | | Product Categories: | | | Mol File: | 124412-00-6.mol |  |
| | 5-CFDA, AM Chemical Properties |
| Boiling point | 737.0±60.0 °C(Predicted) | | density | 1.51±0.1 g/cm3(Predicted) | | form | Solid | | color | White to off-white | | Appearance | Solid Powder |
| | 5-CFDA, AM Usage And Synthesis |
| Description | 5-carboxyfluorescein diacetate acetoxymethyl ester is a cell-permeant fluorogenic esterase substrate. It can be converted by nonspecific esterases of living cells from a membrane-permeable, nonpolar, nonfluorescent substance to a polar product CF, indicating the integrity of the plasma membrane. | | Uses | 5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1]. | | References | [1] Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21. DOI:10.1016/j.mimet.2012.03.005 |
| | 5-CFDA, AM Preparation Products And Raw materials |
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