4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

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4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Basic information
Product Name:4-Nitrophenyl phosphate bis(cyclohexylammonium) salt
Synonyms:phosphoricacid,mono(4-nitrophenyl)ester,compd.withcyclohexanamine(1:2);PNP-PHOS 2 CHX;P-NITROPHENYL PHOSPHATE DICYCLOHEXYLAMINE SALT;P-NITROPHENYL PHOSPHATE DI(CYCLOHEXYLAMMONIUM) SALT;p-nitrophenyl dihydrogen phosphate, compound with cyclohexylamine (1:2);P-NITROPHENYL PHOSPHATE*DI(CYCLOHEXYLAMM ONIUM);4-NITROPHENYLPHOSPHORIC ACID BIS(CYCLOHEXYLAMMONIUM) SALT;4-NITROPHENYL PHOSPHATE BIS(CYCLOHEXYLAMINE) SALT
CAS:52483-84-8
MF:C18H32N3O6P
MW:417.44
EINECS:257-949-0
Product Categories:substrate;Alkaline Phosphatase;Alkaline PhosphataseEnzyme Substrates;Alphabetical ListingELISA Substrates;Detection Substrates;Enzyme Substrates;Powders;Substrates by Enzyme
Mol File:52483-84-8.mol
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Structure
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Chemical Properties
Melting point 199-200°C
storage temp. -20°C
form powder
Water Solubility water: 10mg/mL, clear, colorless to faintly yellow
BRN 6553276
CAS DataBase Reference52483-84-8(CAS DataBase Reference)
EPA Substance Registry SystemPhosphoric acid, mono(4-nitrophenyl) ester, compd. with cyclohexanamine (1:2) (52483-84-8)
Safety Information
Safety Statements 22-24/25
WGK Germany 3
F 8-10-21
TSCA TSCA listed
HS Code 2940000080
MSDS Information
ProviderLanguage
SigmaAldrich English
ALFA English
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Usage And Synthesis
Uses4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.
Biological Activityp-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product th at can be measured spectrophotometrically at 405 nm.
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Preparation Products And Raw materials
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