Arachidonoyl thio-pc manufacturers
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| | Arachidonoyl thio-pc Basic information |
| Product Name: | Arachidonoyl thio-pc | | Synonyms: | 2-DEOXY-2-THIO ARACHIDONOYL PC;2-DEOXY-2-THIOARACHIDONOYLPHOSPHATIDYLCHOLINE;1-hexadecyl-2-arachidonoylthio-2-deoxyglycero-3-phosphorylcholine;1-Hexadecyl-2-arachidonoylthio-2-deoxy-sn-glycero-3-phosphorylcholine;Thiopc;Arachidonoyl thio-PC (substrate);YDSCLGYBUIDZNF-WWBBCYQPSA-N;1-O-HEXADECYL-2-DEOXY-2-THIO-R-(5Z,8Z,11Z,14Z-EICOSATETRAENOYL)-SN-GLYCERYL-3-PHOSPHORYLCHOLINE | | CAS: | 146797-82-2 | | MF: | C44H82NO6PS | | MW: | 784.17 | | EINECS: | | | Product Categories: | | | Mol File: | 146797-82-2.mol |  |
| | Arachidonoyl thio-pc Chemical Properties |
| storage temp. | Store at -20°C | | solubility | DMF: 25 mg/ml; DMSO: 25 mg/ml; Water: 0.5 mg/ml | | form | Liquid | | pka | 1.184±0.50(predicted) | | color | Colorless to light yellow |
| | Arachidonoyl thio-pc Usage And Synthesis |
| Description | Arachidonoyl thio-PC is a substrate for many phospholipase A2s (PLA2s) including sPLA2, cPLA2, and iPLA2. Cleavage of the sn-2 fatty acid by PLA2 results in generation of a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) and DTP to allow quantitation of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by excluding or inhibiting sPLA2 and iPLA2 activities in the assay. | | Uses | Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay. | | References | [1] L YU E A D. Thio-based phospholipase assay.[J]. Methods in enzymology, 1991, 197: 65-75. DOI: 10.1016/0076-6879(91)97133-j |
| | Arachidonoyl thio-pc Preparation Products And Raw materials |
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