| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Proteinase K, recombinant, PCR Grade
Purity:Solution from Pichia pastoris Package:1EA Remarks:3115828001
|
| Company Name: |
JiangSu Star Science Co.,Ltd
|
| Tel: |
400-605-7200 15335295587 |
| Email: |
3941881028@qq.com |
| Products Intro: |
Product Name:Proteinase K, recombinant, PCR Grade
Package:10g; 100g;500g;1kg;5KG;10KG
|
| Company Name: |
Merck KGaA
|
| Tel: |
21-20338288 |
| Email: |
ordercn@merckgroup.com |
| Products Intro: |
Product Name:Proteinase K, recombinant, PCR Grade
|
|
| | Proteinase K, recombinant, PCR Grade Basic information |
| | Proteinase K, recombinant, PCR Grade Chemical Properties |
| storage temp. | 2-8°C | | form | buffered aqueous solution (18 ± mg/mL; pH 7.5) | | Specific Activity | ~2units/mg protein |
| | Proteinase K, recombinant, PCR Grade Usage And Synthesis |
| Uses | - This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
- The enzyme promotes cell lysis by activating a bacterial autolytic factor.
- Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
- The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.
- Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.
| | General Description | Proteinase K is a subtilisin-related serine protease that has no pronounced cleavage specificity.
The enzyme is also available as a lyophilizate. |
| | Proteinase K, recombinant, PCR Grade Preparation Products And Raw materials |
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