FURA-FF, AM manufacturers
- Fura-FF AM
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- $229.00
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2026-07-15
- CAS:348079-12-9
- Purity:
- Supply Ability: 10g
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| | FURA-FF, AM Basic information |
| Product Name: | FURA-FF, AM | | Synonyms: | Fura-FF, AM [Fura-2FF, AM] *CAS 348079-12-9*;FURA-FF, AM;5-Oxazolecarboxylic acid, 2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-2,3-difluorophenoxy]ethoxy]-2-benzofuranyl]-, (acetyloxy)methyl ester | | CAS: | 348079-12-9 | | MF: | C43H43F2N3O24 | | MW: | 1023.8 | | EINECS: | | | Product Categories: | | | Mol File: | 348079-12-9.mol |  |
| | FURA-FF, AM Chemical Properties |
| Boiling point | 971.5±75.0 °C(Predicted) | | density | 1.447±0.06 g/cm3(Predicted) | | storage temp. | Store at -20°C | | solubility | DMSO: soluble | | form | A solid | | pka | 0.36±0.50(Predicted) | | color | Light yellow to yellow |
| | FURA-FF, AM Usage And Synthesis |
| Description | Fura-FF AM is a cell-permeable acetoxymethyl ester of the fluorescence calcium indicator fura-FF (potassium salt) . As fura-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce fura-FF. Fura-FF is a difluorinated derivative of the calcium indicator fura-2 . Unlike, fura-2, fura-FF has negligible magnesium sensitivity, thus reducing interference from this cation.1 Fura-FF also has a higher calcium dissociation constant than fura-2 (Kd(calcium) = 6 and 0.14 μM, respectively).1,2 However, the spectral properties of fura-FF and fura-2 are similar with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium, with a shift to 339/507 nm in the presence of a high calcium concentration.3 Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high concentrations of calcium, such as mitochondria, or in cell systems that have relatively low calcium buffering capacities, such as neuronal dendrites and spines.4,5,6 | | Uses | Fura-FF AM is a cell-permeable acetoxymethyl ester of fura-FF, a dluorescent calcium indicator. Fura-FF AM is hydrolyzed by intracellular esterases to release fura-FF in cells. Fura-FF is a difluorinated derivative of the calcium indicator fura-2. Compared to fura-2, fura-FF has a low affinity for calcium and is suitable for studying compartments with high concentrations of calcium. | | References | 1. Hyrc, K.L., Bownik, J.M., and Goldberg, M.P. Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC Cell Calcium 27(2),75-86(2000).
2. Grynkiewicz, G., Poenie, M., and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties J. Biol. Chem. 260(6),3440-3450(1985).
3. Ruggiu, A.A., Bannwarth, M., and Johnsson, K. Fura-2FF-based calcium indicator for protein labeling Org. Biomol. Chem. 8(15),3398-3401(2010).
4. Aponte, Y., Bischofberger, J., and Jonas, P. Efficient Ca2+ buffering in fast-spiking basket cells of rat hippocampus J. Physiol. 586(8),2061-2075(2008).
5. Canepari, M., Vogt, K., and Zecevic, D. Combining voltage and calcium imaging from neuronal dendrites Cell. Mol. Neurobiol. 28(8),1079-1093(2008).
6. Marcu, R., Neely, C.K., Karamanlidis, G., et al. Multi-parameter measurement of the permeability transition pore opening in isolated mouse heart mitochondria J. Vis. Exp. 67:4131,(2012). |
| | FURA-FF, AM Preparation Products And Raw materials |
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