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Sigma-Aldrich |
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Alfa Chemistry |
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| | Cholesta-3,5-diene Basic information |
| | Cholesta-3,5-diene Chemical Properties |
| Melting point | 78-80 °C (lit.) | | Boiling point | 433.93°C (rough estimate) | | density | 0.9250 | | refractive index | 1.4922 (estimate) | | storage temp. | -20°C | | form | Solid | | color | White to off-white | | biological source | natural (organic) | | InChIKey | RLHIRZFWJBOHHD-HKQCOZBKSA-N | | SMILES | CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C=CCC[C@]4(C)[C@H]3CC[C@]12C |
| Hazard Codes | Xn | | Risk Statements | 22 | | Safety Statements | 36 | | WGK Germany | 3 | | RTECS | FZ5600000 | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Acute Tox. 4 Oral |
| | Cholesta-3,5-diene Usage And Synthesis |
| Uses | Cholesta-3,5-diene was used as standard in HPLC in quality analysis of different types of olive oils and edible fats. | | Definition | ChEBI: Cholesta-3,5-diene is a cholestanoid. | | Biochem/physiol Actions | Cholesta-3,5-diene is an oxysterol, a cholesterol derivative by auto-oxidation. Oxysterols are non-genomic regulators of cholesterol homeostasis. The biological effects include protein prenylation, apoptosis, modulation of sphingolipid metabolism and platelet aggregation. Oxysterols bind to liver X receptors, modulate cholesterol efflux and decrease the uptake of cholesterol by the cells. | | in vivo | Neutrophil Recruitment: Cholesta-3,5-diene (30 μg/ml; topical application; single dose) promotes neutrophil recruitment to tailfin wounds in transgenic zebrafish larvae (Tg(mpx:GFP)) by 6 h post-treatment1.
Tissue Regeneration: Cholesta-3,5-diene (30 μg/ml; topical application; single dose) does not enhance tissue regeneration in zebrafish tailfin wounds, unlike the lipid fraction Ft containing Cholesta-3,5-diene1.
| Animal Model: | Transgenic zebrafish larvae (Tg(mpx:GFP), 4 days post-fertilization, male/female mixed, 200-300 mg)[1] | | Dosage: | 30 μg/mL Cholesta-3,5-diene (dissolved in ethanol, diluted in E2 medium) | | Administration: | Topical application to transected tailfin immediately after wounding, single dose | | Result: | Significantly increased neutrophil accumulation at the wound site by 6 h post-treatment (2-fold vs. vehicle control.
No effect was observed in uninjured tailfins[1]. |
| Animal Model: | Transgenic zebrafish larvae (Tg(mpx:GFP), 4 days post-fertilization, male/female mixed, 200-300 mg)1 | | Dosage: | 30 μg/mL Cholesta-3,5-diene (dissolved in ethanol, diluted in E2 medium) | | Administration: | Topical application to transected tailfin immediately after wounding, single dose | | Result: | Did not promote significant tissue regeneration at 2 or 6 days post-treatment, unlike the lipid fraction Ft (which showed dose-dependent regeneration).
Resulted maximum regeneration with Ft of 50 μg/mL.
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| | Cholesta-3,5-diene Preparation Products And Raw materials |
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