Dios-Arg (trifluoroacetate salt) manufacturers
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| | Dios-Arg (trifluoroacetate salt) Basic information |
| Product Name: | Dios-Arg (trifluoroacetate salt) | | Synonyms: | Dios-Arg (trifluoroacetate salt);(3β,25R)-3-[6-[[(2S)-2-amino-5-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate]spirost-5-en-3-ol,bis(2,2,2-trifluoroacetate) | | CAS: | 1807353-31-6 | | MF: | C41H66F3N5O7 | | MW: | 798 | | EINECS: | | | Product Categories: | | | Mol File: | 1807353-31-6.mol |  |
| | Dios-Arg (trifluoroacetate salt) Chemical Properties |
| storage temp. | Store at -20°C | | solubility | DMF: 30 mg/ml; DMSO: 30 mg/ml; Ethanol: 30 mg/ml; PBS (pH 7.2): 1 mg/ml |
| | Dios-Arg (trifluoroacetate salt) Usage And Synthesis |
| Description | Dios-Arg is a steroid-based cationic lipid that contains a diosgenin skeleton coupled to an L-arginine head group.1 It forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio greater than or equal to 4.2 Dios-Arg facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence and absence of fetal bovine serum, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein . It has been used, coupled to 1,2-dioleoyl-sn-glycero-3-PE (DOPE; ), to bind siRNA and plasmid DNA to for cationic lipid nanoparticles (LNPs) for intracellular transport. Dios-Arg is cytotoxic to H1299 and HeLa cells (IC50s = 83.5 and 74.1 μg/ml, respectively).2 | | Uses | Dios-Arg diTFA, a cationic lipid with an arginine-bearing headgroup, shows obvious double bond proton signals at around 5.2-5.3 ppm. Dios-Arg diTFA is used, coupled to DOPE, to bind siRNA and plasmid to for cationic LNPs for intracellular transport[1][2]. | | References | 1. Sheng, R., Zhuang, X., Wang, Z., et al. Cationic nanoparticles assembled from natural-based steroid lipid for improved intracellular transport of siRNA and pDNA Nanomaterials (Basel) 6(4),(2016).
2. Sheng, R., Wang, Z., Luo, T., et al. Skeleton-controlled pDNA delivery of renewable steroid-based cationic lipids, the endocytosis pathway analysis and intracellular localization Int. J. Mol. Sci. 19(2),(2018). |
| | Dios-Arg (trifluoroacetate salt) Preparation Products And Raw materials |
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