2H-Cho-Arg (trifluoroacetate salt) manufacturers
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| | 2H-Cho-Arg (trifluoroacetate salt) Basic information |
| Product Name: | 2H-Cho-Arg (trifluoroacetate salt) | | Synonyms: | 2H-Cho-Arg (trifluoroacetate salt);(3β)-[6-[[(2S)-2-amino-(5α)-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate],cholestan-3-ol,bis(2,2,2-trifluoroacetate);2H-Cho-Arg (TFA) | | CAS: | 1609010-59-4 | | MF: | C41H72F3N5O5 | | MW: | 772.05 | | EINECS: | | | Product Categories: | | | Mol File: | 1609010-59-4.mol |  |
| | 2H-Cho-Arg (trifluoroacetate salt) Chemical Properties |
| storage temp. | Store at -20°C | | solubility | DMF: 50 mg/ml; DMSO: 50 mg/ml; Ethanol: 50 mg/ml; Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/ml | | form | Liquid | | color | Colorless to light yellow |
| | 2H-Cho-Arg (trifluoroacetate salt) Usage And Synthesis |
| Description | 2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection.1 It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein , but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml). | | Uses | 2H-Cho-Arg (TFA) is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection. | | References | 1. Sheng, R., Wang, Z., Luo, T., et al. Skeleton-controlled pDNA delivery of renewable steroid-based cationic lipids, the endocytosis pathway analysis and intracellular localization Int. J. Mol. Sci. 19(2),(2018). |
| | 2H-Cho-Arg (trifluoroacetate salt) Preparation Products And Raw materials |
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