5-羧基荧光素二乙酸酯
| 中文名称 | 5-羧基荧光素二乙酸酯 |
|---|---|
| 中文同义词 | 5-羧基荧光素二醋酸盐;5-羧基荧光素双醋酸盐;5-羧基荧光素二乙酸酯;5-羧基荧光素 二醋酸;5-CARBOXYFLUORESCEIN DIACETATE 5-羧基荧光素二醋酸盐;5-CFDA (5-CARBOXYFLUORESCEIN DIACETATE),FLUOROGENIC NON-SELECTIVE ESTERASE底物;5-羧基荧光素二乙酸酯,10 MM DMSO 溶液;5(6)-Carboxyfluorescein diacetate, solution, DMSO solution |
| 英文名称 | 5-Carboxyfluorescein diacetate |
| 英文同义词 | 5-Carboxy-di-O-acetylfluorescein, 5-CFDA;5-FAM DA;3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid;5-Carboxyfluorescein diacetate;3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid;5-CFDA [5-Carboxyfluorescein diacetate];5-Carboxyfluorescein diacetate≥ 95% (HPLC);BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC) |
| CAS号 | 79955-27-4 |
| 分子式 | C25H16O9 |
| 分子量 | 460.39 |
| EINECS号 | |
| 相关类别 | 血液学和组织学;染色剂和染料;其它;生化试剂;marker;Aromatics;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Fluorescent;Xanthene |
| Mol文件 | 79955-27-4.mol |
| 结构式 |  |
5-羧基荧光素二乙酸酯 性质
| 熔点 | >250°C |
|---|---|
| 沸点 | 693.1±55.0 °C(Predicted) |
| 密度 | 1.57±0.1 g/cm3(Predicted) |
| 储存条件 | Sealed in dry,Store in freezer, under -20°C |
| 溶解度 | 可溶于DMSO |
| 酸度系数(pKa) | 6.4(at 25℃) |
| 形态 | 固体 |
| 颜色 | 无色固体 |
| 酸碱指示剂变色ph值范围 | Weak green ' uorescence (6.0) to strong green ' uourescence (7.2) |
| 最大波长(λmax) | <300nm |
| BRN | 68265 |
| 主要应用 | 荧光探针、细菌测量方法、酵母细胞内pH检测、微生物细胞外和细胞内pH测定、活细胞检测、癌细胞鉴定、微生物活力评估方法、肿瘤组织增殖活性、角质形成细胞生长测量、试剂、神经退行性疾病、抗肿瘤药物 |
| 生物领域应用 | Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity |
| InChI | 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
| InChIKey | WPUZGNPQMIWOHE-UHFFFAOYSA-N |
| SMILES | CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1 |
5-CFDA(5-Carboxyfluorescein diacetate)是一种可渗入细胞并被水解为5-carboxyfluorescein，用于标记人椎间盘细胞以便体外荧光显微镜观察。
用途
一种重要的染色剂,渗透完整细胞膜的非荧光疏水剂。在细胞中，5- 羧基荧光素二乙酸酯被酶水解成亲水剂。安全信息
| 危险品标志 | T |
|---|---|
| 危险类别码 | 45-46-23/24/25-36/37/38 |
| 安全说明 | 53-22-26-36/37/39-45 |
| WGK Germany | 3 |
| F | 10-21 |
| 海关编码 | 32049010 |
| 存储类别 | 11 - 可燃固体 |
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026/09/15 | C2859 | 5-羧基荧光素二醋酸盐 5-Carboxyfluorescein Diacetate | 79955-27-4 | 50mg | 250元 |
| 2026/09/15 | C2859 | 5-羧基荧光素二醋酸盐 5-Carboxyfluorescein Diacetate | 79955-27-4 | 200mg | 900元 |