3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt manufacturers
- AF647-NHS ester
-
- $891.00 / 1mg
-
2026-01-05
- CAS:407627-60-5
- Min. Order:
- Purity:
- Supply Ability: 10g
|
| | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Basic information |
| Product Name: | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt | | Synonyms: | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt;BP Fluor 647 NHS Ester;AF647 NHS;OKF 647 SE;BP fluor 647 NHS ester trisodium;3-(2-(5-(3,3-Dimethyl-5-sulfo-1-(3-sulfopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3-methyl-5-sulfo-3H-indol-1-ium-1-yl)propane-1-sulfonate | | CAS: | 407627-60-5 | | MF: | C40H49N3O16S4 | | MW: | 956.08 | | EINECS: | | | Product Categories: | | | Mol File: | 407627-60-5.mol | ![3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Structure](CAS/20210111/GIF/407627-60-5.gif) |
| | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Chemical Properties |
| solubility | Soluble in water, DMSO, DMF, etc. | | Appearance | Dark blue solid | | ex/em | 651/672 nm | | ε(extinction coefficient) | 261,000 L⋅mol−1⋅cm−1 | | Φ(quantum yield) | 0.33 | | InChIKey | PIQXJQCAOCUQOD-UHFFFAOYSA-N | | SMILES | C(C1(C(=[N+](CCCS([O-])(=O)=O)C2=CC=C(S(O)(=O)=O)C=C12)C=CC=CC=C1C(C2=CC(S(O)(=O)=O)=CC=C2N1CCCS(O)(=O)=O)(C)C)C)CCCCC(=O)ON1C(CCC1=O)=O |
| | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Usage And Synthesis |
| Description | BP Fluor 647 NHS ester (Alexa Fluor?? 647 NHS Ester equivalent) is an amine reactive bright green-fluorescent dye, it can be labeled to biomolecules directly such as primary antibody, secondary antibody, peptide, etc.
The conjugates can be used as a probe for flow cytometry, immunocytochemical assays, including In-protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development. Alexa Fluor?? is registered trademarks of Thermo Fisher Scientific. | | Chemical Properties | Appearance: Dark blue solid
ex/em: 651/672 nm
Extinction coefficient (ε): 261,000 L??mol??1??cm??1
Quantum yield (Φ): 0.33 | | Uses | AF647-NHS ester is an analog of Alexa Fluor 647 (AF647). AF647 is a far-red fluorescent dye with an excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). Storage: Protect from light[1][2]. | | References | [1] Fernandez A, et al. In vivo characterization of the biodistribution profile of amphipol A8-35. J Membr Biol. 2014;247(9-10):1043-1051. DOI:10.1007/s00232-014-9682-8
[2] Karlsson J K G, Laude A, Hall M J, et al. Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy[J]. Chemistry–A European Journal, 2019, 25(65): 14983-14998. |
| | 3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt Preparation Products And Raw materials |
|