重组人糖原磷酸化酶

中文名称

【GPBB】重组人糖原磷酸化酶

英文名字

Recombinant Human Glycogen Phosphorylase

供应商

ProSpec

产品货号

ENZ-282

背景资料

Glycogen phosphorylase is one of the phosphorylaseenzymes（EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule， and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism， it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.
Glycogen phosphorylase can only act on linearchainsof glycogen（a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch（which are exceedingly common in glycogen). In these situations， a debranching enzymeis necessary， which will straighten out the chain in that area. Additionally， an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done， glycogen phosphorylase can continue.
An insulinstimulated enzyme known as phosphoprotein phosphatase（PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.
GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.

序列

该【GPBB】重组人糖原磷酸化酶Recombinant Human Glycogen Phosphorylase是Prospec品牌 产品，具体的蛋白序列可查看提供的英文版本产品说明书。

来源宿主

大肠杆菌中重组表达

溶解建议

提供的人糖原磷酸化酶（GPBB）产品为过滤灭菌的浓度为0.8 mg/ml无色溶液；其中每毫克人糖原磷酸化酶（GPBB）产品溶解于50%甘油溶液中.

保存建议

人糖原磷酸化酶（GPBB）溶液可以在10℃保存7天；如果想延长保存时间，建议分装后置于-18℃冻存.请避免反复冻融.

其他

ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。 领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。