产品名称：Zymolyase-20T / Zymolyase-100T
品牌：Nacalai
Zymolyase.pdf
Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit
Product Information
There are two preparations of Zymolyase®, Zymolyase®-20T and Zymolyase®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase®-20T is ammonium sulfate precipitate while Zymolyase®-100T is a further purified preparation by affinity chromatography(9). Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions(6-8). Further informations related to Zymolyase® can be obtained in the reference section below(12-16).
Product Name | Zymolyase®-20T | Zymolyase®-100T |
Form | Lyophilized Powder |
Purification | Ammonium Sulfate Precipitation | Affinity Chromatography |
Activity | 20,000 units/g | 100,000 unites/g |
Essential enzyme | β-1,3-glucan laminaripentaohydrolase |
Other activities
contained (※1) | β-1,3-glucanase | approx. 1.5 x 106 units/g | approx. 1.0 x 107 units/g |
protease | approx. 1.0 x 104 units/g | approx. 1.7 x 104 units/g |
mannanase | approx. 1.0 x 106 units/g | approx. 6.0 x 104 units/g |
Contaminants | Amylase | Trace amount | Non detectable |
Xylanase |
Phosphatase |
Optimum pH and Temp. | pH7.5, 35°C (for lysis of viable yeast cells) |
pH6.5, 45°C (for hydrolysis of yeast glucan) |
Stability | 2°C | No loss of activity was found after storage for 1 year |
Heat stability | 30°C | 70% of the lytic activity is lost after storage for 3 months |
90% of the lytic activity is lost after storage for 3 months |
60°C | Lytic activity is lost on incubation for 5 minutes |
Specificity (Lytic Spectrum) | Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc. |
(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.
Ordering Information
Product Name | Storage | Product No. | PKG Size | Price |
Zymolyase®-20T | R | 07663-91 | 1 g | 请咨询客服 |
07663-04 | 10 g |
Zymolyase®-100T | R | 07665-55 | 500 mg | 请咨询客服 |
07665-84 | 5 g |
Zymolyase® is a registered trademark of Kirin Holdings Company, Limited
(Storage) R: Refrigerator
关键字： Zymolyase-20T;Zymolyase-100T;Zymolase酶;Nacalai;
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