基本信息
产品名称：Cdk4 Antibody
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用：WB, IF/ICC, IHC
特异性：Cdk4
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：Recombinant Cdk4 expressed in E.coli (AA 6-295)
阳性对照：WB: HeLa, MCF7, Hep G2, A549, A431, Ramos, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, lung cancer and lymphoma tissues. IF/ICC: HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：多克隆抗体
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Antigen affinity purified + Protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂：0.05% Proclin 300
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
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Western Blot: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - Western Blot
All lanes: Cdk4 Antibody (Ab095764) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 34 kDa
Observed band size: 33kDa
Exposure time: 2.2 s
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IF/ICC: Cdk4 Antibody (Ab095764)
Cdk4 Antibody (Ab095764) - IF/ICC
IF analysis of Cdk4 Antibody (Ab095764) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Cdk4 Antibody (Ab095764) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab095764.html
关键字： 细胞周期蛋白依赖性激酶4多克隆抗体 ; Cdk4抗体;Cdk 4 antibody ; cdk4 antibody
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