基本信息
产品名称:AMPK alpha 2 Mouse mAb
规格或纯度:ExactAb™, Validated, Carrier Free, 0.5 mg/mL
应用:WB, IF/ICC
特异性:PRKAA2
种属反应性:人(Human)
免疫原:Recombinant protein of human PRKAA2 (AA 49-394).
阳性对照:WB: HEK293, HeLa, MCF7 and Hep G2 cell lysates. IF: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:单克隆抗体
克隆号:206CT5.4.2
Format:Whole IgG
亚型:Mouse IgG2a
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein G purified
物理外观:Liquid
储存缓冲液:10mM PBS, 0.09% Sodium azide, pH7.4.
防腐剂:0.09% Sodium azide
浓度:0.5 mg/mL
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(2 周)。-20°C下长期保存(12个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human)
克隆号:206CT5.4.2
偶联:Unconjugated
图片

>
Western Blot: AMPK alpha 2 Mouse mAb (Ab088664)
AMPK alpha 2 Mouse mAb (Ab088664) - Western Blot
All lanes: AMPK alpha 2 Mouse mAb (Ab088664) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 62 kDa
Observed band size: 65 kDa
Exposure time: 15.1 s

>
IF/ICC: AMPK alpha 2 Mouse mAb (Ab088664)
AMPK alpha 2 Mouse mAb (Ab088664) - IF/ICC
Fluorescent image of HeLa cell stained with AMPK alpha 2 (Ab088664). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AMPK alpha 2 Mouse mAb (Ab088664) (1/25, 1 h at 37℃). For secondary antibody, AF488 conjugated donkey anti-mouse antibody (green) was used (1/400, 50 min at 37℃. Cytoplasmic actin was counterstained with AF555 (red) conjugated Phalloidin (7units/mL, 1 h at 37℃). AMPK alpha 2 immunoreactivity is localized to Cytoplasm significantly.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab088664.html



关键字: 腺苷单磷酸活化蛋白激酶α2抗体 ; AMPKa2抗体 ; PRKAA2抗体 ; AMPK α2 小鼠单克隆抗体
上海阿拉丁生化科技股份有限公司是A股上市公司((股票代码:688179),专注于科研试剂的研发、生产和销售,已陆续建立多个工厂和研发中心。作为领军企业,阿拉丁始终坚持质量第一,连续13年被评为“最受欢迎试剂品牌”。
阿拉丁目前常备库存试剂产品品种超过7万种,SKU总数超过46万,产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域,是国内少数化学试剂到生物试剂全面发展的国产试剂品牌,产品同步发布在我们国内外电商平台。