基本信息
产品名称：Recombinant Cytokeratin 20 Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
应用：WB, IF/ICC, IP, Flow Cytometry, IHC
特异性：Cytokeratin 20
种属反应性：人(Human)
预测反应种属：大鼠(Rat), 普通狨(Common marmoset), 山羊(Goat), 猪(Pig)
交叉反应：Human
免疫原：A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20.
阳性对照：WB: HT-29 cell lysate. IHC: Colonic adenocarcinoma tissue. Flow: HT-29 cells. ICC/IF: HT-29 cells. IP: HT-29 whole cell lysate.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
克隆号：RR648
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐剂：0.01% Sodium azide
浓度：0.5 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human)
克隆号：RR648
偶联：Unconjugated
图片
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Western Blot: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 0.1 s
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IF/ICC: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IF/ICC
Recombinant Cytokeratin 20 Antibody (Ab098925) staining Cytokeratin 20 in HT-29 cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant Cytokeratin 20 Antibody (Ab098925) (1/200) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500).
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Flow Cytometry : Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - Flow Cytometry
Overlay histogram showing HT-29 cells stained with Recombinant Cytokeratin 20 Antibody (Ab098925) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Cytokeratin 20 Antibody (Ab098925) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Blue) was used as a control.
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IHC: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colonic adenocarcinoma tissue labelling Cytokeratin 20 with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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IP: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IP
Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/50 dilution.
2nd Ab: G&R HRP for IP 1/500
Lane 1: Recombinant Cytokeratin 20 Antibody (Ab098925) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Cytokeratin 20 Antibody (Ab098925) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30.0 s.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab098925.html
关键字： anti- Cytokeratin 20抗体 ; 细胞角蛋白20抗体 ; 重组细胞角蛋白20抗体;CD20 antibody
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