基本信息
产品名称:Recombinant GSK3 beta Antibody
规格或纯度:重组, ExactAb™, 已验证, 高性能, 见COA
应用:WB, IF/ICC, IHC
特异性:GSK3 beta
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)
免疫原:A synthetic peptide derived from human GSK3 beta (AA 1-13)
阳性对照:WB: Jurkat, HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human cholangiocarcinoma. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂:0.01% Sodium aizde
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)
偶联:Unconjugated
图片

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Western Blot: Recombinant GSK3 beta Antibody (Ab106628)
Recombinant GSK3 beta Antibody (Ab106628) - Western Blot
All lanes: Recombinant GSK3 beta Antibody (Ab106628) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 10.7 s

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IHC: Recombinant GSK3 beta Antibody (Ab106628)
Recombinant GSK3 beta Antibody (Ab106628) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GSK3 beta Antibody (Ab106628). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
IF analysis of Recombinant GSK3 beta Antibody (Ab106628) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GSK3 beta Antibody (Ab106628) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab106628.html



关键字: 糖原合酶激酶3beta抗体 ; 糖原合酶激酶3beta单克隆抗体 ; 糖原合酶激酶3beta重组单克隆抗体 ; GSK3 beta抗体
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