基本信息
产品名称：HA tag Mouse mAb
规格或纯度：ExactAb™, Validated, Carrier Free, 1.0 mg/mL
应用：WB, IF/ICC
特异性：HA
种属反应性：不依赖种属(Species independent)
免疫原：Synthetic peptide HA-tag.
阳性对照：WB: Over-expressed HA-tagged protein in HEK293T cell lysate. IF/ICC: HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62 #PPL00549-2b.
偶联：Unconjugated
备注：本产品即将售完下架，替代产品为 Ab106781
产品属性
克隆类型：单克隆抗体
克隆号：4G3
Format：Whole IgG
亚型：Mouse IgG1
SDS-PAGE：150 kDa
纯化方法：Antigen affinity purified
物理外观：Liquid
储存缓冲液：10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.2
防腐剂：0.02% Sodium azide
浓度：1.0 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（12个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：小鼠(Mouse)
种属反应性：不依赖种属(Species independent)
克隆号：4G3
偶联：Unconjugated
图片
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Western Blot: HA tag Mouse mAb (Ab106776)
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s
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Western Blot: HA tag Mouse mAb (Ab106776)
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 30.0 s
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IF/ICC: HA tag Mouse mAb (Ab106776)
HA tag Mouse mAb (Ab106776) - IF/ICC
IF image of HA tag Mouse mAb (Ab106776) stained HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62. The cells were 4% para-formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1 h to permeabilise the cells and block non-specific pro-tein-protein interactions. The cells were then incubated with the HA tag Mouse mAb (Ab106776) (1/200) and the antibody Flag-tag pAb(1/200) at 5 µg/mL overnight at 4°C. The secondary anti-body (Red) was Goat anti-Mouse IgG (H+L)-TRITC and the secondary antibody (Green) was Goat anti-Rabbit IgG (H+L)-FITC used at 1/1000 dilution for 1 h. Hoechst33342 was used to stain the cell nuclei (blue).
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab106776.html
关键字： HA tag 标签单克隆抗体 ; HA tag 单克隆抗体 ; HA tag 抗体 ; HA 标签小鼠 mAb
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