基本信息
产品名称:PARK7 Antibody
规格或纯度:无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用:WB, IF/ICC, IHC
特异性:PARK7
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:Recombinant PARK7 expressed in E.coli (AA 1-189).
阳性对照:WB: Jurkat, MCF7, Hep G2, HeLa, HUVEC and NIH/3T3 cell lysates. IHC: Human spleen, kidney, skeletal muscle tissues. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Antigen-specific affinity chromatography followed by Protein A affinity chromatography
物理外观:Liquid
储存缓冲液:10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4.
防腐剂:0.05% Proclin 300
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片

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Western Blot: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - Western Blot
All lanes: PARK7 Antibody (Ab120346) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
Exposure time: 15.0 s

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Spleen Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Skeletal muscle Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IHC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Skeletal muscle Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IF/ICC: PARK7 Antibody (Ab120346)
PARK7 Antibody (Ab120346) - IF/ICC
IF analysis of PARK7 Antibody (Ab120346) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PARK7 Antibody (Ab120346) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab120346.html



关键字: PARK7抗体 ; 帕金森氏病蛋白7抗体 ; PARK7 抗体;CAP1 antibody
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