基本信息
产品名称:SGK1 Antibody
规格或纯度:ExactAb™, Validated, Carrier Free, 0.5 mg/mL
应用:WB, IF/ICC
特异性:SGK1
种属反应性:人(Human), 小鼠(Mouse)
免疫原:A KLH conjugated synthetic peptide from the human region of human SGK (Ser78) (AA 70-103).
阳性对照:WB: Jurkat, A431 and Hep G2 cell lysates.
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Protein A purified + Peptide affinity purified
物理外观:Liquid
储存缓冲液:10mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂:0.09% Sodium azide
浓度:0.5 mg/mL
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse)
偶联:Unconjugated
图片

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Western Blot: SGK1 Antibody (Ab127341)
SGK1 Antibody (Ab127341) - Western Blot
All lanes: SGK1 Antibody (Ab127341) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 53 kDa
Exposure time: 32.8 s

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IF/ICC: SGK1 Antibody (Ab127341)
SGK1 Antibody (Ab127341) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SGK with SGK1 Antibody (Ab127341) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at
1/100 dilution (red). The nuclear counter stain is DAPI (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab127341.html



关键字: 糖皮质激素调节激酶1多克隆抗体 ; 糖皮质激素调节激酶1抗体 ; SGK1抗体;OTTHUMP00000017247 antibody
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