基本信息
产品名称:STAT5a Antibody
规格或纯度:无载体, ExactAb™, 已验证, 高性能, 见COA
应用:WB, IF/ICC, IHC
特异性:STAT5a
种属反应性:人(Human), 小鼠(Mouse), 猪(Pig)
免疫原:Recombinant STAT5a expressed in E.coli (AA 673-773)
阳性对照:WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Antigen affinity purified and Protein A purified
物理外观:Liquid
储存缓冲液:10 mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂:0.02% Sodium azide
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 猪(Pig)
偶联:Unconjugated
图片

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Western Blot: STAT5a Antibody (Ab129464)
STAT5a Antibody (Ab129464) - Western Blot
All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 24.2 s

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IHC: STAT5a Antibody (Ab129464)
STAT5a Antibody (Ab129464) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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IF/ICC: STAT5a Antibody (Ab129464)
STAT5a Antibody (Ab129464) - IF/ICC
IF analysis of STAT5a Antibody (Ab129464) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with STAT5a Antibody (Ab129464) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab129464.html



关键字: 信号转导和转录激活因子5a多克隆抗体 ; STAT5a 抗体;Mammary gland factor antibody ; MGF antibody
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