基本信息
产品名称：AKT1 Mouse mAb
规格或纯度：ExactAb™, Validated, Carrier Free, 1.0mg/mL
应用：WB, IF/ICC, Flow Cytometry, ELISA
特异性：AKT1
应用：WB,Flow,ELISA,IF,ICC
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 猴(Monkey)
免疫原：Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
阳性对照：WB: Recombinant human AKT1 (aa 1-150); MCF7, NIH/3T3, COS-7 and C6 cell lysates; AKT1 (aa 1-150)-hIgGFc transfected HEK-293 whole cell lysate. ICC/IF: HepG2 cells. Flow: HeLa cells.
偶联：Unconjugated
产品属性
抗体类型：Primary antibody
克隆类型：单克隆抗体
克隆号：1F7E10
Format：Whole IgG
亚型：IgG1
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein G purified
来源：Ascitic fluid
物理外观：Liquid
储存缓冲液：Purified antibody in PBS with 0.05% sodium azide.
防腐剂：0.05% sodium azide
浓度：1.0mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
功能和特点
宿主种属：小鼠(Mouse)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 猴(Monkey)
克隆号：1F7E10
偶联：Unconjugated
图片
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Western Blot: AKT1 Mouse mAb (Ab087937)
AKT1 Mouse mAb (Ab087937) - Western Blot
All lanes: AKT1 Mouse mAb (Ab087937) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 55.7 kDa
Observed band size: 55.1 kDa
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Flow Cytometry: AKT1 Mouse mAb (Ab087937)
AKT1 Mouse mAb (Ab087937) - Flow Cytometry
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AKT1 with AKT1 Mouse mAb (Ab087937) at 1/200 dilution (green) compared with a negative control (red).
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ICC/IF: AKT1 Mouse mAb (Ab087937)
AKT1 Mouse mAb (Ab087937) - ICC/IF
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AKT1 (green) using AKT1 Mouse mAb (Ab087937) in ICC/IF. Blue: DRAQ5 DNA dye. Red: Actin filaments stained with AlexaFluor®-555-Phalloidin.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab087937.html
关键字： AKT1 小鼠单克隆抗体 ; AKT1 小鼠单抗;EC 2.7.11.1 ; PKB
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