基本信息
产品名称：beta Actin Mouse mAb
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用：WB, IF/ICC, Flow Cytometry
特异性：beta actin
应用：WB,Flow,ICC/IF,ELISA
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
预测反应种属：鸡(Chicken), 牛(Cow), 狗(Dog), 猪(Pig), 兔(Rabbit)
免疫原：Synthetic peptide in beta Actin
阳性对照：WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells.
偶联：Unconjugated
产品属性
抗体类型：Primary antibody
克隆类型：单克隆抗体
克隆号：FG01/2D10
Format：Whole IgG
亚型：IgG1
轻链亚型：kappa
纯化方法：Protein A purified
来源：Hybridoma supernatant
物理外观：Liquid
储存缓冲液：PBS, pH 7.2
防腐剂：No
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：小鼠(Mouse)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号：FG01/2D10
偶联：Unconjugated
图片
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Western Blot: beta Actin Mouse mAb (Ab155822)
beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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ICC/IF: beta Actin Mouse mAb (Ab155822)
beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
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Flow Cytometry: beta Actin Mouse mAb (Ab155822)
beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab155822.html
关键字： β actin antibody ; β-actin antibody ; beta Actin 抗体 ; Actin 抗体
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