Biotinylated Human ALPG / ALPPL2 Protein, His,Avitag™ (MALS verified)
分子别名(Synonym)
ALPG,GCAP,ALP-1,ALPPL2,PLAP-like,ALPPL,Alkaline phosphatase, germ cell type
表达区间及表达系统(Source)
Biotinylated Human ALPG Protein, His,Avitag (ALG-H52E1) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Asp 503 (Accession # P10696-1).
Predicted N-terminus: Ile 20
蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 56.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

背景(Background)
ALPPL2在幼稚多能干细胞中特异性高表达。此外,在幼稚状态体细胞重编程、幼稚启动转化和其他系统中,使用ALPPL2启动子驱动的荧光报告系统可以特异性地指示幼稚状态多能性的建立或撤销,从而将ALPPL2鉴定为幼稚状态多能干性。特定的细胞表面标记。一些研究发现,敲除ALPPL2可以特异性地影响幼稚状态多能性的建立、稳定性和维持,证明ALPPL2蛋白是一种功能性的幼稚状态表面分子标记。
关键字: ALPG;ALPG蛋白;ALPG重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。