| 名称 | Phorbol 12-myristate 13-acetate |
| 描述 | Phorbol 12-myristate 13-acetate (PMA) is a natural phorbol ester that acts as an activator of PKC, SphK, and NF-κB, and is commonly used to induce differentiation of THP-1 cells and establish dermatitis models. |
| 细胞实验 | αT3-1 and LβT-2 cells are grown in monolayer cultured in DMEM in humidified incubator 5% CO2 at 37°C. Serum starvation is with 0.1% FCS in the same medium for 16 h. GnRH and PMA are then added for the length of time as indicated. In general, αT3-1 cells are transiently transfected by ExGen 500 or by jetPRIME, while LβT2 cells only by jetPRIME transfection reagent. For experiments with dominant-negative (DN) PKCs, αT3-1 cells (in 6 cm plates) are transfected with 1.5 μg of p38α-GFP with 3 μg of control vector, pCDNA3, or with 3 μg of the DN-PKCs constructs. For LβT2 cells, transfections are performed (in 10 cm plates) with 4 μg of p38α-GFP along with 9 μg of control vector, pCDNA3, or with 9 μg of the DN-PKCs constructs. Approximately 30 h after transfection, the cells are serum-starved (0.1% FCS) for 16 h and later stimulated with GnRH or PMA, washed twice with ice-cold PBS, treated with the lysis buffer, followed by one freeze-thaw cycle. Cells are harvested; following centrifugation (15,000×g, 15 min, 4°C) supernatants are taken for immunoprecipitation experiments [2]. |
| 动物实验 | All experiments are performed with male Wistar rats (weighing 250-280 g). One hundred and thirty-five Wistar rats are randomly divided into seven groups. (1) Rats in the sham group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline; (2) Rats in the IR group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline 30 min before middle cerebral artery occlusion (MCAO); (3) Rats in the Carbenoxolone (CBX) group (n=21) are given a lateral cerebral ventricle injection of CBX (5 μg/mL×10 μL) 30 min before MCAO; (4) Rats in the Sch-6783 group (n=21) are given a lateral cerebral ventricle injection of DZX (2 mM×30 μL) 30 min prior to MCAO; (5) Rats in the 5-HD group (n=21) are given a lateral cerebral ventricle injection of 5-HD (100 mM×10 μL), and after 10 min, DZX is injected 15 min prior to MCAO; (6) The rats in the DZX + Ro group (n=15) are given a lateral cerebral ventricle injection of DZX, and after 10 min, Ro-31-8425 (400 μg/kg) is injected 15 min prior to MCAO; (7) The rats in the 5-HD+PMA group (n=15) are given an intraperitoneal injection of PMA (200 μg/kg) after the injection of 5-HD and DZX [3]. |
| 体外活性 | 方法：球体培养的人类黑色素瘤细胞 WM 系列用 Phorbol 12-myristate 13-acetate (50 ng/mL) 处理 3 天，使用 MTS 方法检测细胞生长情况。
结果：Phorbol 12-myristate 13-acetate 促进黑色素瘤细胞增殖，WM35 细胞的细胞数提高到 265%。[1]
方法：人单核白血球细胞 THP-1 用 Phorbol 12-myristate 13-acetate (200 ng/mL) 处理 1-5 天，使用光学显微镜评估形态，使用 Flow Cytometry 方法检测靶点表达。
结果：Phorbol 12-myristate 13-acetate 诱导 THP-1 细胞分化为巨噬细胞样细胞 (THP-1 巨噬细胞)，CD11 和 CD14 的细胞表面表达增加。[2]
方法：人静脉内皮细胞 HUVECs 用 Phorbol 12-myristate 13-acetate (10-40 ng/mL) 处理 8 h，使用 Wound healing migration assay 检测细胞迁移情况。
结果：短期 Phorbol 12-myristate 13-acetate 处理可增强内皮细胞迁移。[3] |
| 体内活性 | 方法：为研究佛波酯对啮齿类动物大脑发育的影响，将 Phorbol 12-myristate 13-acetate (100-500 μg/kg) 单次腹腔注射给药给缺乏 IL-18 或 IRAK-4 的新生大鼠和小鼠，24 h、7 天或14 天后处死动物。
结果：Phorbol 12-myristate 13-acetate 在大脑中诱导炎症反应并引起广泛的神经退行性变。缺乏 IL-18 或 IRAK-4 对 Phorbol 12-myristate 13-acetate 诱导的脑损伤有保护作用。[4]
方法：为构建急性小鼠耳炎症模型，将 Phorbol 12-myristate 13-acetate (20 μL 125 μg/mL PMA 丙酮溶液) 局部处理 CD-1 小鼠双耳，风干并完全吸收。
结果：用 Phorbol 12-myristate 13-acetate 攻击的耳组织在施用约 2 小时开始出现炎症迹象，包括肿胀和发红。[5] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 60 mg/mL (97.27 mM), Sonication is recommended.
H2O : Insoluble
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 6 mg/mL (9.73 mM), Solution.
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| 关键字 | TPA | SphK | Sphingosine kinase | S1PReceptor | S1P Receptor | Protein kinase C | PKC | Phorbol myristate | Phorbol 12-myristate 13-acetate | Phorbol 12myristate 13acetate | Phorbol 12-myristate | Phorbol 12 myristate 13 acetate | Nuclear factor-κB | Nuclear factor-kappaB | NF-κB | NFκB | NF-kB | NFkB | Inhibitor | inhibit |
| 相关产品 | D-Psicose | Uridine | Sodium propionate | Ethyl palmitate | 1,4-Naphthoquinone | Glucosamine | Undecane | Fumaric acid | Kojic acid | Magnesium sulfate | Ethyl linoleate | N,N-Dimethylacetamide |
| 相关库 | 经典已知活性库 | 已知活性化合物库 | 激酶抑制剂库 | 抗衰老化合物库 | 抗癌天然产物库 | 天然产物库 | 中药单体化合物库 | GPCR靶点分子库 | TGF-β/Smad靶点化合物库 | 抗癌临床化合物库 | 高通量筛选天然产物库 | 抗癌药物库 |