| 名称 | Decitabine |
| 描述 | Decitabine (Deoxycytidine) is a deoxycytidine analog, a DNA methyltransferase inhibitor with oral activity. Decitabine has antitumor activity and antimetabolic activity. Decitabine induces cell cycle arrest and apoptosis. |
| 细胞实验 | For cell cycle analysis, KARPAS-299 cells were incubated for 24 h with 1 μM of 5-aza-CdR in RPMI and grown for 4 days in fresh RPMI only. Then, 105–106 cells were suspended in 500 μl PI-buffer (0.1% Na–citrate dihydrate, 0.1% Triton X-100, 0.1% RNAse (DNAse free) in PBS). Propidium–iodide (ROTH, dissolved in PBS) was added to a concentration of 10 μg/ml and the cells were incubated for 30 min at 37 °C. The analysis was performed on a flow cytometer using the BD FACS Diva Software. Three independent samples of 5-aza-CdR treated and PBS controls were analyzed. Descriptive statistics for analysis are reported as mean ± SEM [4]. |
| 激酶实验 | The rate of DNA synthesis was measured by the incorporation of radioactive thymidine into DNA. HL-60 (5 × 10^3 cells/ml) and KG1a cells (10^4 cells/ml) were suspended in 2 ml RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 h (drugs were added simultaneously). At 48 h, 0.5 μCi [3H] thymidine (6.7 Ci/mmol) was added to each well and incubated for an additional 24 h. The cells were placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA were then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using scintillation counter. The IC50 is defined as the concentration of drug that inhibits by 50% the DNA synthesis of the leukemic cell lines from the dose-response curve [1]. |
| 动物实验 | For xenografts, NOD.CB17-Prkdc?scid/NCrHsd (NOD/SCID, Harlan Laboratories) mice were used. KARPAS-299 human cells were grown as described above, dissolved in sterile PBS to a concentration of 1×107 cells/ml and inoculated subcutaneously (1×10^6 cells/injection) into the right and left flanks of the mice. Tumor range was followed measuring tumor length and tumor width with a calliper. Mice weighed approximately 25 g at the beginning of the therapy. 5-Aza-CdR was dissolved in sterile PBS and was administered intraperitoneally (i.p.). Each mouse received 2.5 mg/kg/mouse per treatment. Control mice were administered 100 μl of sterile PBS. Therapies were adjusted regarding start and duration of the treatment in order to obtain optimal treatment procedures. In schedule A, three mice were treated with 5-aza-CdR 11 days after inoculation, when tumor size was approximately 1 cm2. The control group contained two mice. The mice received 5-aza-CdR or PBS every day for eight days. In schedule B, two mice were treated with 5-aza-CdR three days after inoculation and three mice five days after inoculation when tumors were not or just palpable. 5-Aza-CdR was administered every other day for five times to each mouse. The control group contained two mice [4]. |
| 体外活性 | 方法：人急性白血病细胞 molt4 用 Decitabine (0.00625-100 μM) 处理 24-96 h，使用 CCK-8 方法检测细胞增殖。
结果：Decitabine 以剂量和时间依赖的方式抑制 molt4 细胞的增殖，处理 72 h 和 96 h 的 IC50 分别为 84.461 μM 和 10.113 μM。[1]
方法：人 BCP-ALL 细胞 SEM 和 RS4;11 用 Decitabine (1000 nM) 处理 72 h，使用 Flow Cytometry 检测细胞周期情况。
结果：Decitabine 引起 SEM 细胞中 G0/G1 停滞。RS4;11 的细胞周期不受 Decitabine 的影响。[2] |
| 体内活性 | 方法：为检测体内抗肿瘤活性，将 Decitabine (0.4 mg/kg) 腹腔注射给携带 ALL 肿瘤 SEM-ffluc-GFP 或 RS4;11-ffluc-GFP 的 NSG 小鼠，每天一次，持续三十天。
结果：Decitabine 显著延迟了 SEM-ffluc-GFP 和 RS4-ffluc 衍生的异种移植物模型中的白血病细胞增殖。[2]
方法：为检测体内抗肿瘤活性，将 Decitabine (0.8 mg/kg) 腹腔注射给携带人胆管癌肿瘤 TFK-1 的 Balb-c nu/nu 小鼠，每天一次，持续十四天。
结果：在 TFK-1 小鼠异种移植物中，Decitabine 延缓了荷瘤小鼠的肿瘤生长并提高了其存活率。[3] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| 溶解度 | H2O : 11.4 mg/mL (49.95 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (8.76 mM), Sonication is recommended.
DMSO : 55 mg/mL (241.01 mM), Sonication is recommended.
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| 关键字 | NucleosideAntimetabolite | Nucleoside Antimetabolite/Analog | Nucleoside Antimetabolite | NSC-127716 | NSC127716 | Inhibitor | inhibit | DNMTs | DNAMethyltransferase | DNA MTases | DNA methyltransferase (DNMT) | DNA Methyltransferase | Decitabine | Apoptosis | Analog |
| 相关产品 | Formamide | Urea | Sodium Molybdate | Dimethyl phthalate | Aceglutamide | Alginic acid | Cysteamine hydrochloride | Metronidazole | Sildenafil citrate | Citric Acid Triammonium | Stavudine | Tamoxifen |
| 相关库 | 抗癌活性化合物库 | 经典已知活性库 | 抗癌上市药物库 | 已知活性化合物库 | EMA 上市药物库 | FDA上市及药典收录分子库 | 抗衰老化合物库 | FDA 上市药物库 | 抗肺癌化合物库 | 药物功能重定位化合物库 | 抗癌临床化合物库 | 抗癌药物库 |