| 名称 | Bay 11-7085 |
| 描述 | Bay 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM). |
| 细胞实验 | Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.(Only for Reference) |
| 激酶实验 | In Gel Kinase Assay: In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30?°C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control. |
| 体外活性 | 在含Caov-3细胞的无胸腺裸鼠体内,BAY 11-7085可使紫杉醇对腹内传播和腹水产生的抑制作用增强.BAY11-7085对脑膜炎相关的NF-κB活性增加有抑制作用,改善被感染大鼠临床状态,并使脑膜炎相关的CNS并发症和脑膜炎症显著衰减. |
| 体内活性 | BAY 11-7085与紫杉醇联用可增强对NF-κB活性的抑制,并使经紫杉醇处理细胞的存活率降低。通过抑制NF-κB,BAY11-7085可抑制TNFα诱导的粘附分子E-selectin,ICAM-1及VCAM-1的表达。BAY11-7085（10 μM）对HUVEC细胞无细胞毒性。此外,Bay11-7085与LY294002联用可协同影响PEL细胞的凋亡。 |
| 存储条件 | keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 125 mg/mL (501.34 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (8.02 mM), Sonication is recommended.
Ethanol : 24.9 mg/mL (99.87 mM), Sonication is recommended.
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| 关键字 | Nuclear factor-κB | Nuclear factor-kappaB | NF-κB | NFκB | NF-kB | NFkB | IκBα phosphorylation | IκB | Inhibitor | inhibit | IKK | IkB/IKK | IkB | Ferroptosis | BAY 11-7085 | Bay 11-7085 | Bay 117085 | Bay 11 7085 | Apoptosis |
| 相关产品 | L-Cystine | L-Glutamic acid | Cysteamine hydrochloride | Alginic acid | Dextran sulfate sodium salt (MW 5000) | Stavudine | Tributyrin | L-Glutamine | L-Ascorbic acid sodium salt | L-Ascorbic acid | Sodium 4-phenylbutyrate | N,N-Dimethylacetamide |
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