GRB10 (Growth Factor Receptor-Bound Protein 10) is an adaptor protein involved in regulating cellular signaling pathways, particularly those linked to insulin/IGF-1 receptors and tyrosine kinase receptors. It modulates key processes like cell growth, proliferation, and metabolism by interacting with receptors and downstream signaling molecules (e.g., PI3K/AKT, MAPK pathways). GRB10 exhibits tissue-specific imprinting, with preferential expression of paternal alleles in certain tissues, influencing developmental and metabolic functions.
GRB10 antibodies are essential tools for studying its role in these pathways. They enable detection and quantification of GRB10 expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers also use these antibodies to investigate GRB10's interactions with partners (e.g., receptors, kinases) through co-immunoprecipitation assays. Dysregulation of GRB10 has been implicated in diseases such as diabetes, intrauterine growth restriction, and cancer, making its antibodies valuable for exploring molecular mechanisms and therapeutic targets. Specific GRB10 isoforms (e.g., cytoplasmic vs. membrane-bound) may require isoform-specific antibodies. Validation using knockout controls ensures antibody specificity, critical for reliable experimental outcomes in both basic and translational research contexts.