ANAPC4抗体,Rabbit Polyclonal ANAPC4 Antibody
  • ANAPC4抗体,Rabbit Polyclonal ANAPC4 Antibody
  • ANAPC4抗体,Rabbit Polyclonal ANAPC4 Antibody
  • ANAPC4抗体,Rabbit Polyclonal ANAPC4 Antibody

Rabbit Polyclonal ANAPC4 Antibody

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:ANAPC4抗体英文名称:Rabbit Polyclonal ANAPC4 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 150 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: ANAPC4
2025-05-09 ANAPC4抗体 Rabbit Polyclonal ANAPC4 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 150 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/2000 Human,Mouse,Rat
   

产品详情

AliasesAPC4
WB Predicted band size92 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human ANAPC4
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HepG2 cells, 231 cells, hela cells, Primary antibody: P04286(ANAPC4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04286(ANAPC4 Antibody) at dilution 1/50. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P04286(ANAPC4 Antibody) at dilution 1/50. (Original magnification: ×200)    


           

参考文献

以下是关于ANAPC4抗体的3-4篇参考文献的示例(注:文献信息为示例性质,可能需根据实际研究调整):

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1. **标题**: *APC/C Subunit ANAPC4 is Essential for Cell Cycle Progression*

**作者**: Yamamoto S, et al.

**摘要**: 研究利用ANAPC4抗体进行免疫印迹和免疫荧光实验,发现敲除ANAPC4会导致细胞周期停滞在G1期,证实其在APC/C复合体介导的泛素化降解中的核心作用。

2. **标题**: *Structural and Functional Analysis of ANAPC4 in the APC/C Complex*

**作者**: Zhang M, et al.

**摘要**: 通过ANAPC4抗体进行免疫共沉淀和质谱分析,揭示了ANAPC4与其他APC/C亚基的相互作用,阐明其维持复合体结构完整性的关键功能。

3. **标题**: *ANAPC4 Dysregulation in Cancer: A Role in Genomic Instability*

**作者**: Chen L, et al.

**摘要**: 使用ANAPC4抗体检测肿瘤组织样本,发现其表达水平与染色体不稳定性相关,提示ANAPC4缺失可能通过破坏APC/C功能促进癌症进展。

4. **标题**: *ANAPC4 Antibody-based Screening for Drug Resistance in Yeast*

**作者**: Gupta P, et al.

**摘要**: 开发了一种基于ANAPC4抗体的高通量筛选方法,发现靶向APC/C活性的化合物可逆转酵母模型中的化疗耐药表型。

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**注意**:以上文献为示例,实际引用需根据具体研究检索PubMed、Google Scholar等数据库获取。推荐使用关键词“ANAPC4 antibody”、“APC/C complex”或结合具体研究领域(如癌症、细胞周期)进行精准搜索。

       

背景信息

The ANAPC4 antibody targets the Anaphase-Promoting Complex subunit 4 (ANAPC4), a critical component of the Anaphase-Promoting Complex/Cyclosome (APC/C). APC/C is a multi-subunit E3 ubiquitin ligase that regulates cell cycle progression by targeting specific proteins for proteasomal degradation, ensuring timely transitions through key phases like metaphase-to-anaphase and exit from mitosis. ANAPC4. also known as APC4. plays a structural and regulatory role in maintaining APC/C stability and substrate recognition. It facilitates interactions between the core complex and co-activators (CDC20 or CDH1), which are essential for activating APC/C's ubiquitination activity toward substrates such as cyclin B1 and securin.

ANAPC4 antibodies are widely used in research to study cell cycle dynamics, protein degradation mechanisms, and mitotic regulation. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ANAPC4 expression, localization, and interactions in various biological contexts. Dysregulation of APC/C function, including ANAPC4 abnormalities, has been linked to cancers, genomic instability, and developmental disorders. Researchers also use these antibodies to explore therapeutic targets, as modulating APC/C activity could influence cell proliferation in diseases like cancer. Validation of ANAPC4 antibodies often involves knockout cell lines or tissue models to confirm specificity, ensuring reliable detection in both physiological and pathological studies.

       
关键字: ANAPC4抗体;ANAPC4;ANAPC4 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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