APEH (Acylaminoacyl-peptide hydrolase) antibody is a tool used to study the APEH enzyme, a serine hydrolase involved in protein quality control and metabolic regulation. APEH, also known as acylpeptide hydrolase or oxidized protein hydrolase, catalyzes the removal of N-acetylated amino acids from peptides, facilitating protein degradation and recycling. It plays roles in clearing oxidatively damaged proteins, linking it to cellular stress responses and aging-related disorders like Alzheimer’s disease. Structurally, APEH features an α/β-hydrolase fold with a catalytic triad (Ser, Asp, His).
APEH antibodies are widely employed in research to detect protein expression, localization, and activity via techniques like Western blotting, immunohistochemistry, and ELISA. They aid in exploring APEH's involvement in cancer (where its expression varies in tumors), neurodegeneration, and redox homeostasis. Some antibodies target specific domains or post-translational modifications, enabling functional studies. Commercial APEH antibodies are typically raised in rabbits or mice, available as monoclonal or polyclonal forms. Validation includes testing knockout controls and activity assays. Recent studies highlight APEH's therapeutic potential, driving demand for reliable antibodies to probe its mechanisms in disease models and drug development.